ANTIBODY-RESPONSE TO THE M2-PROTEIN OF INFLUENZA A-VIRUS EXPRESSED IN INSECT CELLS
ANTIBODY-RESPONSE TO THE M2-PROTEIN OF INFLUENZA A-VIRUS EXPRESSED IN INSECT CELLS
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DOI：
10.1099/0022-1317-74-1-143
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发表时间：
1993-01-01
影响因子：
3.8
通讯作者：
KENDAL, AP
中科院分区：
文献类型：
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作者：
BLACK, RA;ROTA, PA;KENDAL, AP
构建了表达流感A/Ann Arbor/6/60(H_2N_2)病毒(AA60病毒)M2蛋白的重组杆状病毒。表达的M2蛋白可被抗M2蛋白的单抗识别，并与AA60病毒感染细胞的M2蛋白在SDS-聚丙烯酰胺凝胶上共游走。免疫荧光研究表明，表达的M2蛋白存在于感染重组杆状病毒的果蝇Sf9细胞表面。使用表达的M2蛋白的免疫分析能够在感染A型流感病毒的人和雪貂的血清样本中检测到M2蛋白的抗体。
A recombinant baculovirus expressing the M2 protein from influenza A/Ann Arbor/6/60 (H2N2) virus (AA60 virus) was constructed. The expressed M2 protein was recognized by a monoclonal antibody specific for the M2 protein and comigrated with the M2 protein from cells infected with AA60 virus on SDS-polyacrylamide gels. Immunofluorescence studies indicated that the expressed M2 protein was present on the surface of Spodoptera frugiperda (Sf9) cells infected with the recombinant baculovirus. Immunoassays using the expressed M2 protein were able to detect antibodies to the M2 protein in serum samples from humans and ferrets infected with influenza A viruses.