Laser capture microdissection of Candida albicans from host tissue.
Laser capture microdissection of Candida albicans from host tissue.
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DOI：
10.1007/978-1-61779-539-8_27
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发表时间：
2012
影响因子：
--
通讯作者：
C. Westwater;D. Schofield
中科院分区：
文献类型：
--
作者：
C. Westwater;D. Schofield
Laser microdissection is a technique in which specific populations of cells are acquired from sections of complex tissue under direct microscopic visualization. The technique can be used to selectively harvest or ablate host and/or fungal cells from a variety of biological specimens, including human, animal, or plant tissue sections. When coupled with downstream applications such as proteomic and molecular analyses, laser microdissection can address a variety of important biological questions specifically related to the in vivo host-fungus interaction. In this chapter, we describe how laser microdissection enables researchers to selectively isolateCandida albicanscells from host-infected tissue. Detailed protocols are provided for tissue handling and processing, slide preparation, and laser capture microdissection (LCM). Using these methods, we highlight the use of LCM to examine infection-relatedC. albicansgene expression.
