Dynamic reorganization of photosystem II supercomplexes in response to variations in light intensities
Dynamic reorganization of photosystem II supercomplexes in response to variations in light intensities
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DOI：
10.1016/j.bbabio.2016.06.011
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发表时间：
2016-10-01
影响因子：
4.3
通讯作者：
Pagliano, Cristina
中科院分区：
文献类型：
--
作者：
Albanese, Pascal;Manfredi, Marcello;Pagliano, Cristina
植物是固着生物，需要适应不断变化的光照条件才能生存。这些变化触发了类囊体膜中膜蛋白复合物的动态重组。光系统II（PSII）及其捕光系统（LHCII）是这种驯化反应的主要靶点，越来越多的证据表明，类囊体中PSII-LHCII超复合物的数量和组成会随着光强和光质的变化而动态调整。我们表征了豌豆植物类囊体膜中PSII-LHCII超复合物对不同光强度的长期适应的响应。我们提供了证据的PSII-LHCII超复合物的重组显示其天线部分的明显变化。质谱分析显示，Lhcb 3，Lhcb 6和M-LHCII三聚体结合到PSII核心的特定减少，而Lhcb4.3亚型增加响应于高的光强度。Lhcb蛋白含量的调制与功能PSII天线大小的减少相关。这些结果表明，Lhcb 3，Lhcb4.3和Lhcb 6天线亚基的调制PSII天线的大小后，长期适应增加的光水平的主要球员。在任何光照条件下，在分离的PSII-LHCII超复合物中均未检测到PsbS，尽管高光驯化植物的类囊体中积累增加，表明PsbS不是PSII-LHCII超复合物的组成成分。(C)2016爱思唯尔B. V.保留所有权利。
Plants are sessile organisms and need to acclimate to ever-changing light conditions in order to survive. These changes trigger a dynamic reorganization of the membrane protein complexes in the thylakoid membranes. Photosystem II (PSII) and its light harvesting system (LHCII) are the major target of this acclimation response, and accumulating evidences indicate that the amount and composition of PSII-LHCII supercomplexes in thylakoids are dynamically adjusted in response to changes in light intensity and quality.In this study, we characterized the PSII-LHCII supercomplexes in thylakoid membranes of pea plants in response to long-term acclimation to different light intensities. We provide evidence of a reorganization of the PSII-LHCII supercomplexes showing distinct changes in their antenna moiety. Mass spectrometry analysis revealed a specific reduction of Lhcb3, Lhcb6 and M-LHCII trimers bound to the PSII cores, while the Lhcb4.3 isoform increased in response to high light intensities. The modulation of Lhcb protein content correlates with the reduction of the functional PSII antenna size. These results suggest that the Lhcb3, Lhcb4.3 and Lhcb6 antenna subunits are major players in modulation of the PSII antenna size upon long-term acclimation to increased light levels. PsbS was not detected in the isolated PSII-LHCII supercomplexes at any light condition, despite an increased accumulation in thylakoids of high light acclimated plants, suggesting that PsbS is not a constitutive component of PSII-LHCII supercomplexes. (C) 2016 Elsevier B.V. All rights reserved.