Predicted expansion of the claudin multigene family
Predicted expansion of the claudin multigene family
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DOI：
10.1016/j.febslet.2011.01.028
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发表时间：
2011-02-18
期刊：
影响因子：
3.5
通讯作者：
Tsukita, Sachiko
中科院分区：
文献类型：
--
作者：
Mineta, Katsuhiko;Yamamoto, Yasuko;Tsukita, Sachiko
紧密连接蛋白（Claudins，Cldn）是紧密连接（tightjunction，TJ）的重要膜蛋白，形成细胞旁选择性透过屏障。它们是由一个多基因家族产生的，在小鼠和人类中有24个成员。基于一个全面的搜索结合系统发育分析，我们确定了三个新的claudin（claudin-25，-26，和-27）。定量RT-PCR显示，这三种新的claudins表达的组织和/或发育阶段依赖性的方式。当在培养的MDCK和Eph上皮细胞系中外源表达时，紧密连接蛋白-25和-26，而不是紧密连接蛋白-27，被免疫荧光定位于TJ。这些发现扩展了claudin家族，包括至少27 members.Structured摘要：Claudin-25 and ZO-1 colocalize by fluorescence microscopy（View interaction）ZO-1 and Claudin-26 colocalize by fluorescence microscopy（View interaction）（C）2011 Federation of European Biochemical Societies. Elsevier B. V.出版，保留所有权利。
Claudins (Cldn) are essential membrane proteins of tight junctions (TJs), which form the paracellular permselective barrier. They are produced by a multi-gene family of 24 reported members in mouse and human. Based on a comprehensive search combined with phylogenetic analyses, we identified three novel claudins (claudin-25, -26, and -27). Quantitative RT-PCR revealed that the three novel claudins were expressed in a tissue- and/or developmental stage-dependent manner. Claudins-25 and -26, but not claudin-27, were immunofluorescently localized to TJs when exogenously expressed in cultured MDCK and Eph epithelial cell lines. These findings expand the claudin family to include at least 27 members.Structured summary:Claudin-25 and ZO-1 colocalize by fluorescence microscopy (View interaction)ZO-1 and Claudin-26 colocalize by fluorescence microscopy (View interaction) (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.