Isolation and identification of two galangin metabolites from rat urine and determination of their in vitro hypolipidemic activity
Isolation and identification of two galangin metabolites from rat urine and determination of their in vitro hypolipidemic activity
复制标题
大鼠尿液中两种高良姜素代谢物的分离鉴定及体外降血脂活性测定
DOI：
10.4314/tjpr.v15i6.16
复制
发表时间：
2016-06-01
影响因子：
0.6
通讯作者：
Tan, Yinfeng
中科院分区：
文献类型：
--
作者：
Zhang, Xuguang;Cheng, Shouqian;Tan, Yinfeng
Purpose: To investigate the lipid-lowering activity of two metabolites of galangin, namely, galangin-3-O-beta-D-glucuronic acid (GG-1) and galangin-7-O-beta-D-glucuronic acid (GG-2).Methods: Female Sprague-Dawley rats were orally administered with galangin. The two metabolites of galangin were isolated from urine sample and purified using Sephadex LH-20 and semi-preparative high performance liquid chromatography (HPLC). The structures of the metabolites were identified by analyzing spectroscopic data. Hypolipidemic activity was evaluated in HepG2 cells. The down- or up-regulation of lipogenic genes was detected using real-time quantitative polymerase chain reaction (qPCR).Results: Both metabolites of galangin showed hypolipidemic activity. These activities are closely associated with the down-regulation of lipogenic genes such as SREBP-1a, SREBP-1c, and SREBP-2 transcription factors, and the downstream genes such as FAS, ACC, and HMGR were revealed by real-time qPCR data.Conclusion: The results show that both metabolites possess better lipid-lowering activities than galangin. These hypolipidemic activities are closely associated with inhibiting key genes or proteins that regulated the biosynthesis of both cholesterol and triglycerides.
