One-Step Purification of Catechol 2,3-dioxygenase from Bacillus cereusZL1
One-Step Purification of Catechol 2,3-dioxygenase from Bacillus cereusZL1
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蜡样芽孢杆菌 ZL1 中儿茶酚 2,3-双加氧酶的一步纯化
DOI：
10.4028/www.scientific.net/amr.955-959.455
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Shuang Zhu
中科院分区：
文献类型：
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作者：
Lin Zhou;Zishan Zhou;Shuang Zhu
用一步色谱法纯化了蜡样芽孢杆菌ZL1中催化苯酚降解中间裂解第二步的关键酶——邻苯二酚2,3-双加氧酶（C23O）。根据生物量和C23O活性分析优化培养条件，并采用SDS-PAGE法监测分离过程。纯化的C23O分子量为37±3KDa，在DEAE-Sepharose Fast Flow上采用线性梯度模式，用400 mmol NaCL磷酸钾缓冲液洗脱活性酶。
Catechol 2,3-dioxygenase (C23O), the key enzyme catalyzing the second step in the phenol degradation meta-cleavage pathway has been purified to homogeneity by one-step chromatography from bacterial strain Bacillus cereus ZL1. The culture condition was optimized according to the analysis of the biomass and C23O activity, and the separation process was monitored by SDS-PAGE method. The molecular weight of the purified C23O was 37±3KDa and the active enzyme was eluted by potassium phosphate buffer with 400 mmol NaCL using linear gradient mode on DEAE-Sepharose Fast Flow.
不动杆菌YAA苯胺降解途径中儿茶酚2,3-双加氧酶的纯化和表征及其抗底物抑制的突变酶。
DOI：
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发表时间：
2007
期刊：
Bioscience Biotechnology Biochemistry 71
影响因子：
--
作者：
T.Takeo;M.Nishimura;M.Shirai;H.Takahashi;and S.Negoro.
通讯作者：
and S.Negoro.