Quantitative profiling of phosphatidylcholine and phosphatidylethanolamine in a steatosis/fibrosis model of rat liver by nanoflow liquid chromatography/tandem mass spectrometry
Quantitative profiling of phosphatidylcholine and phosphatidylethanolamine in a steatosis/fibrosis model of rat liver by nanoflow liquid chromatography/tandem mass spectrometry
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DOI：
10.1016/j.chroma.2008.04.031
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发表时间：
2008-06-13
影响因子：
4.1
通讯作者：
Moon, Myeong Hee
中科院分区：
文献类型：
--
作者：
Ahn, Eun Jeong;Kim, Hanna;Moon, Myeong Hee
采用四氯化碳(CCl4)诱导的大鼠肝脏脂肪变性/纤维化模型，对磷脂酰胆碱(PC)和磷脂酰乙醇胺(PE)进行了定量分析。用纳米流液相色谱-电喷雾电离串联质谱仪(NLC/ESI/MS/MS)分析大鼠肝脏样品(每周3次，每次0.5mLCCl4/kg，连续30、60、90d)中完整的磷脂混合物，并用碰撞诱导解离法鉴定37种PC和19种PE。定量分析采用具有内标的多重标准加入法，并考虑了不同PC物种的MS峰强度与其碳链长度的关系以供校正。结果发现，随着CCl4的加入，PC和PE物种的总量显著减少。虽然大多数PC和PE物种的浓度都有很大程度的下降，但3种PE和7种PC的表达上调了一倍以上。(C)2008爱思唯尔B.V.保留所有权利。
Quantitative analysis of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was carried out using a steatosis/fibrosis model of rat livers, which were induced by a chronic administration of carbon tetrachloride (CCl4). Intact phospholipid mixtures from each liver sample (from rats fed with 0.5 mL CCl4/kg three times a week for 30, 60, and 90 days) were analyzed by nanoflow liquid chromatography-electrospray ionization tandem mass spectrometry (nLC/ESI/MS/MS), and identifications of 37 PC and 19 PE species were made by collision-induced dissociation. The quantitative analysis utilized a multiple standard addition method with an internal standard, and the relationship between the MS peak intensities of different PC species and their carbon chain length was included for calibration. It was found that the total amount of PC and PE species decreased significantly with administration of CCl4. While concentrations of most PC and PE species decreased to a great extent, three PEs and seven PCs were up-regulated more than twofold. (C) 2008 Elsevier B.V. All rights reserved.