FAST AXONAL-TRANSPORT IN EXTRUDED AXOPLASM FROM SQUID GIANT-AXON
FAST AXONAL-TRANSPORT IN EXTRUDED AXOPLASM FROM SQUID GIANT-AXON
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DOI：
10.1126/science.6183745
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发表时间：
1982-01-01
期刊：
影响因子：
56.9
通讯作者：
ALLEN, RD
中科院分区：
文献类型：
--
作者：
BRADY, ST;LASEK, RJ;ALLEN, RD
视频增强对比度差分干涉光学显微镜的发展，允许研究的顺行和逆行快速轴突运输的膜细胞器在鱿鱼巨大的轴突。这个过程通常持续数小时后，轴质被挤出的巨大轴突和轴突质膜的限制。在缺乏质膜或其他渗透性屏障的制备物中跟踪全范围的膜性细胞器（30 nm囊泡至5000 nm线粒体）的运动。轴突的快速运输不需要质膜。动作电位不参与快速转运的调节。轴质周围的渗透性屏障的情况下，使其成为膜细胞器运输的直接生化，药理学和物理操作的重要模型。
Development of video-enhanced contrast-differential interference contrast for light microscopy has permitted study of orthograde and retrograde fast axonal transport of membranous organelles in the squid giant axon. This process continues normally for hours after the axoplasm is extruded from the giant axon and removed from the confines of the axonal plasma membrane. The movements of the full range of membranous organelles (30 nm vesicles to 5000 nm mitochondria) are followed in a preparation that lacks a plasma membrane or other permeability barrier. The plasma membrane is not required for fast axonal transport. Action potentials are not involved in the regulation of fast transport. The absence of a permeability barrier surrounding the axoplasm makes this an important model for direct biochemical, pharmacological and physical manipulations of membranous organelle transport.