Involvement of the linker histone H1Foo in the regulation of oogenesis
Involvement of the linker histone H1Foo in the regulation of oogenesis
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DOI：
10.1530/rep-21-0233
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发表时间：
2022-08-01
期刊：
影响因子：
3.8
通讯作者：
Aoki, Fugaku
中科院分区：
文献类型：
--
作者：
Funaya, Satoshi;Kawabata, Yuria;Aoki, Fugaku
简而言之：在卵母细胞中，染色质结构在其生长过程中被疏松，这似乎是建立能力以完成成熟和受精后的进一步发展所必需的。摘要：在卵母细胞发生过程中，卵母细胞表现出独特的分裂方式和基因表达模式。染色质结构被认为参与了这些过程的调节。在这项研究中，我们研究了连接子组蛋白在卵子发生过程中调节高阶染色质结构的功能。由于H1foo在卵母细胞中高表达，我们用siRNA敲除了H1foo，并观察了卵母细胞的生长、成熟和受精。然而，H1foo基因敲除对这些过程中的任何一个都没有影响。H1b或H1d的过度表达，它具有高浓缩染色质的能力，在卵母细胞中低水平表达，导致染色质收紧，降低卵母细胞成熟的成功率。相比之下，H1a的过度表达并不影响生长或成熟。H1a在卵母细胞中高水平表达，但压缩染色质的能力较低。因此，在H1foo基因敲除的卵母细胞中，H1a，而不是其他变体，可能会补偿H1foo的功能。这些结果表明，H1foo参与了松散染色质结构的形成，这是卵母细胞成熟所必需的。此外，体细胞连接蛋白变异体的低表达，例如H1b和H1d，对于松散的染色质和减数分裂进程是重要的。
In brief: In oocytes, chromatin structure is loosened during their growth, which seems to be essential for the establishment of competence to accomplish the maturation and further development after fertilization. This paper shows that a linker histone variant, H1foo, is involved in the formation of loosened chromatin structure in growing oocytes.Abstract: During oogenesis, oocytes show a unique mode of division and gene expression patterns. Chromatin structure is thought to be involved in the regulation of these processes. In this study, we investigated the functions of linker histones, which modulate higher-order chromatin structure during oogenesis. Because H1foo is highly expressed in oocytes, we knocked down H1foo using siRNA and observed oocyte growth, maturation, and fertilization. However, H1foo knockdown had no effect on any of these processes. Overexpression of H1b or H1d, which has a high ability to condense chromatin and is expressed at a low level in oocytes, resulting in tightened chromatin and a decreased success rate of oocyte maturation. By contrast, overexpression of H1a, which is expressed at a high level in oocytes and has a low ability to compact chromatin, did not affect growth or maturation. Therefore, H1a, but not other variants, might compensate for the function of H1foo in H1foo-knockdown oocytes. These results implicate H1foo in the formation of loose chromatin structure, which is necessary for oocyte maturation. In addition, the low expression of somatic linker histone variants, for example, H1b and H1d, is important for loosened chromatin and meiotic progression.