Passive protection against rotavirus-induced diarrhea by monoclonal antibodies to surface proteins vp3 and vp7
Passive protection against rotavirus-induced diarrhea by monoclonal antibodies to surface proteins vp3 and vp7
复制标题
DOI：
10.1128/jvi.58.2.700-703.1986
复制
发表时间：
1986-05
影响因子：
5.4
通讯作者：
P. Offit;R. Shaw;H. Greenberg
中科院分区：
文献类型：
--
作者：
P. Offit;R. Shaw;H. Greenberg
针对两种轮状病毒表面蛋白（VP3和VP7）以及轮状病毒内衣壳蛋白（VP6）的单克隆抗体进行了测试，以保护乳鼠免受强毒轮状病毒攻击的能力。猴轮状病毒RRV株VP7两个不同表位的单克隆抗体在体外中和RRV，并被动保护乳鼠对抗RRV攻击。针对猪轮状病毒OSU株VP3的单克隆抗体在体外中和了三种不同的血清型（OSU，RRV和UK），并被动保护乳鼠免受OSU，RRV和UK病毒诱导的腹泻。在开发轮状病毒基因克隆疫苗时，应考虑vp3在引起对异型轮状病毒攻击的保护中的作用。或者，用含有来自两种抗原性不同的轮状病毒亲本的vp3和vp7的重组轮状病毒进行免疫接种，可能会防止由两种或更多种轮状病毒血清型诱导的腹泻。
Monoclonal antibodies directed against two rotavirus surface proteins (vp3 and vp7) as well as a rotavirus inner capsid protein (vp6) were tested for their ability to protect suckling mice against virulent rotavirus challenge. Monoclonal antibodies to two distinct epitopes of vp7 of simian rotavirus strain RRV neutralized RRV in vitro and passively protected suckling mice against RRV challenge. A monoclonal antibody directed against vp3 of porcine rotavirus strain OSU neutralized three distinct serotypes in vitro (OSU, RRV, and UK) and passively protected suckling mice against OSU, RRV, and UK virus-induced diarrhea. The role of vp3 in eliciting protection against heterotypic rotavirus challenge should be considered when developing a vaccine with cloned rotavirus genes. Alternatively, immunization with a reassortant rotavirus containing vp3 and vp7 from two antigenically distinct rotavirus parents might protect against diarrhea induced by two or more rotavirus serotypes.