In-gel SOD assay reveals SOD-2 is the single active, water-soluble SOD enzyme in C. elegans.
In-gel SOD assay reveals SOD-2 is the single active, water-soluble SOD enzyme in C. elegans.
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SOD-2是C.优雅的
DOI：
10.1080/10715762.2021.1979228
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发表时间：
2021-06
影响因子：
3.3
通讯作者：
中科院分区：
文献类型：
--
作者：
线虫C.秀丽隐杆线虫有五个sod基因，是需氧生物中最大的一个。早期的研究表明，五个SOD基因中的每一个都能够在异源表达系统中产生完全有活性的SOD蛋白，因此没有一个是假基因。然而，整个sod基因的缺失对C.除了这些动物外，秀丽隐杆线虫似乎对同时施加的氧化应激条件更敏感。我们想知道，在C.通过使用凝胶内SOD活性分析和通过使用KCN作为针对Cu-ZnSOD酶的选择性抑制剂，在这里，我们提供的证据表明，在五种SOD蛋白中，只有线粒体SOD在C的水溶性组分中具有活性。秀丽隐杆线虫提取物的SOD活性明显低于D. melanogaster和E.杆菌我们没有机会测试SOD-4a同工型的活性，其可能是SOD的膜结合形式。突变分析进一步证实，在两种线粒体SOD蛋白中，SOD-2是C.优雅的
The nematode C. elegans has a contingent of five sod genes, one of the largest among aerobic organism. Earlier studies revealed each of the five sod genes is capable of making perfectly active SOD proteins in heterologous expression systems therefore none appears to be a pseudogene. Yet deletion of the entire contingent of sod genes fails to impose any effect on the survival of C. elegans except these animals appear more sensitive to extraneously applied oxidative stress condition. We asked how many of the five sod genes are actually making active SOD enzymes in C. elegans through usage of in-gel SOD activity analysis and by using KCN as a selective inhibitor against Cu-ZnSOD enzyme(s). Here we provide evidence that out of the five SOD proteins only the mitochondrial SOD is active in the water soluble fraction of C. elegans extracts albeit at an apparently much lower activity than the multiple active SODs in D. melanogaster and E. coli. We had no opportunity to test the activity of Sod-4a isoform which is possibly a membrane bound form of SOD. Mutant analysis further confirmed that among the two mitochondrial SOD proteins, SOD-2 is the only naturally active SOD in C. elegans.
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影响因子：
62.1
作者：
Sheng, Yuewei;Abreu, Isabel A.;Cabelli, Diane E.;Maroney, Michael J.;Miller, Anne-Frances;Teixeira, Miguel;Valentine, Joan Selverstone
通讯作者：
Valentine, Joan Selverstone
DOI：
10.1073/pnas.252342899
发表时间：
2002-12-10
影响因子：
11.1
作者：
Kirby, K;Hu, JG;Phillips, JP
通讯作者：
Phillips, JP
影响因子：
3.3
作者：
Corsi AK;Wightman B;Chalfie M
通讯作者：
Chalfie M
影响因子：
4.5
作者：
Van Raamsdonk, Jeremy M.;Hekimi, Siegfried
通讯作者：
Hekimi, Siegfried
影响因子：
4.8
作者：
Honda, Y;Honda, S
通讯作者：
Honda, S