Comparison of the yields of translocations and dicentrics measured using conventional Giemsa staining and chromosome painting
Comparison of the yields of translocations and dicentrics measured using conventional Giemsa staining and chromosome painting
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DOI：
10.1080/095530096145445
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发表时间：
1996-06-01
影响因子：
2.6
通讯作者：
Hayata, I
中科院分区：
文献类型：
--
作者：
Kanda, R;Hayata, I
关于易位和双中心的产量用染色体涂画测量的不一致的结果已经报道。这种不一致可能是由于由于绘画不适合识别着丝粒位置而将双着丝粒错误地标记为易位。本研究利用人外周血淋巴细胞对辐照后首次有丝分裂的染色体畸变进行了分析。当通过绘画和常规吉姆萨染色（定位着丝粒最可靠的方法）分析相同的中期时，观察到易位和双着丝粒的频率相同。
Inconsistent results have been reported regarding the yields of translocations and dicentrics measured using chromosome painting. This inconsistency may be due to mis-scoring of dicentrics as translocations because painting is unsuited to identify centromere positions. In the present study, chromosome aberrations exclusively in the first mitosis after irradiation were analyzed using human peripheral lymphocytes. When identical metaphases were analyzed by both painting and conventional Giemsa staining, which is the most reliable method to locate centromeres, equal frequencies of translocations and dicentrics were observed.