MUTATION IN A TESTIS-SPECIFIC BETA-TUBULIN IN DROSOPHILA - ANALYSIS OF ITS EFFECTS ON MEIOSIS AND MAP LOCATION OF THE GENE
MUTATION IN A TESTIS-SPECIFIC BETA-TUBULIN IN DROSOPHILA - ANALYSIS OF ITS EFFECTS ON MEIOSIS AND MAP LOCATION OF THE GENE
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DOI：
10.1016/0092-8674(80)90481-x
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发表时间：
1980-01-01
期刊：
影响因子：
64.5
通讯作者：
KAUFMAN, TC
中科院分区：
文献类型：
--
作者：
KEMPHUES, KJ;RAFF, EC;KAUFMAN, TC
睾丸特异性β的结构基因-微管蛋白亚基在D.通过显性雄性不育突变B2 tD在遗传学和细胞遗传学上对黑腹果蝇进行了定位，其中睾丸的变体形式β-微管蛋白被表达。B2 t基因在第3染色体遗传图上位于48.5图位单位处，在唾液染色体上位于85 D4 -7带。突变B2 tD导致从减数分裂开始的精子发生的所有阶段中微管功能的破坏。在光学显微镜水平上详细观察了B2 tD基因剂量对减数分裂的影响。在果蝇的睾丸中，表达了变异的微管蛋白亚基，发生异常的减数分裂纺锤体形成、不正确的染色体运动和不能进行胞质分裂。这些微管功能缺陷的程度取决于B2 tD突变的剂量，在突变纯合子的男性中最严重，在突变杂合子的男性中居中，在B2 tD杂合子和含有B2 t基因座的基因组区域的串联重复的男性中最不明显。与微管功能无关的染色体事件，如复制和浓缩，正常发生。在B2 t基因座的映射过程中获得的结果强烈建议单倍不足的网站在或密切联系到这个位点。
The structural gene for a testis-specific .beta.-tubulin subunit in D. melanogaster was mapped genetically and cytogenetically by a dominant male sterile mutation, B2tD, in which a variant form of the testis .beta.-tubulin is expressed. The B2t locus is at 48.5 map units on the 3rd chromosome genetic map, and in bands 85D4-7 on the salivary chromosome map. The mutation B2tD causes disruption of microtubule function in all stages of spermatogenesis, beginning with meiosis. The effects of gene dosage of B2tD on meiosis were examined in detail cytologically at the light microscope level. In testes of flies in which the variant tubulin subunit is expressed, abnormal meiotic spindle formation, improper chromosome movement and failure to undergo cytokinesis occur. The extent of these defects in microtubule function depends on the dosage of the B2tD mutation, being most severe in males homozygous for the mutation, intermediate in males heterozygous for the mutation, and least marked in males heterozygous for B2tD and a tandem duplication of the region of the genome containing the B2t locus. Chromosomal events unrelated to microtubule function, such as replication and condensation, occur normally. Results obtained during mapping of the B2t locus strongly suggest a haplo-insufficient site at or closely linked to this locus.