Reassessment of histone gene expression during cell cycle in human cells by using homologous H4 histone cDNA.
Reassessment of histone gene expression during cell cycle in human cells by using homologous H4 histone cDNA.
复制标题
使用同源 H4 组蛋白 cDNA 重新评估人类细胞细胞周期期间的组蛋白基因表达。
DOI：
10.1073/pnas.76.10.4995
复制
发表时间：
1979
影响因子：
11.1
通讯作者：
G. Stein
中科院分区：
文献类型：
--
作者：
S. Detke;A. Lichtler;I. Phillips;J. Stein;G. Stein
The representation of H4 histone mRNA sequences in RNAs isolated from G1 and S phase HeLa cells was assessed by use of a homologous H4 histone cDNA. S phase cells were obtained by double thymidine block, and G1 cells were obtained by double thymidine block or mitotic selective detachment. Nuclear and cytoplasmic RNAs from S phase cells hybridized with H4 histone cDNA as did nuclear and cytoplasmic RNAs from G1 cells synchronized by double thymidine block. In contrast, significant levels of hybridization were not observed between H4 histone cDNA and nuclear, polysomal, or postpolysomal cytoplasmic RNAs of G1 cells synchronized by mitotic selective detachment. Double thymidine block yields a G1 cell population containing 20-25% S phase cells whereas the G1 population obtained by mitotic detachment contains less than 0.1% S phase cells. The ability of H4 histone cDNA to hybridize with the RNAs from G1 cells obtained after release from double thymidine block can therefore be explained by the presence of S phase cells in such a G1 population--an artifact of the synchronization procedure. We interpret these results to be consistent with the presence of H4 histone mRNA sequences during the S but not G1 phase of the cell cycle in continuously dividing HeLa S3 cells.
