Fos protein expression in mouse hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei upon osmotic stimulus: colocalization with vasopressin, oxytocin, and tyrosine hydroxylase
Fos protein expression in mouse hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei upon osmotic stimulus: colocalization with vasopressin, oxytocin, and tyrosine hydroxylase
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DOI：
10.1016/j.neuint.2004.04.003
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发表时间：
2004-10-01
影响因子：
4.2
通讯作者：
Kiss, A
中科院分区：
文献类型：
--
作者：
Pirnik, Z;Mravec, B;Kiss, A
用免疫组织化学方法研究了小鼠PVN和SON的前、中、后三部分中激活的加压素（AVP）、催产素（OXY）和酪氨酸羟化酶（TH）神经元的数量和分布，在单次注射高渗盐水（HS，400穆尔1.5 M，i. p.）后60分钟。Fos-神经肽双染显示：（1）Fos在室旁核和视上核各部位均有表达，（2）Fos-AVP在室旁核和视上核各部位的表达最多（79个细胞）和Fos-OXY（3）室旁核内Fos-TH胞体数量少，SON内Fos-TH胞体缺失;（4）SON中Fos-AVP和Fos-OXY胞体的相似发生率（约50%）;和（5）在室旁核的室周、室管膜下和室旁核下区中存在激活的AVP、OXY和TH神经元。局部解剖分析显示，大多数表达Fos的AVP神经元位于室旁核中部的背外侧和中央。在PVN的同一部位，Fos-OXY神经元的出现频率相似，但主要分布于PVN的外侧缘和内侧缘沿着。在SON中，Fos-OXY细胞主要分布于背侧，而Fos-AVP细胞主要分布于腹侧。结果表明，HS对室旁核和视上核都不是选择性刺激，室旁核和视上核AVP和OXY细胞在HS介导的信号转导中起重要作用。此外，有限数量的AVP，OXY，TH神经元激活HS可能占其差异功能的专业化选择性应激/渗透压电路激活HS。(C)2004爱思唯尔有限公司保留所有权利。
The quantity and topography of activated vasopressin (AVP), oxytocin (OXY), and tyrosine hydroxylase (TH) neurons were studied immunolustochemically in the anterior, middle, and posterior portions of the PVN and SON in mice 60 min after a single injection of hypertonic saline (HS, 400 mul 1.5 M, i.p.). Fos-neuropeptide double-stainings revealed: (1) Fos expression in each portion of the PVN and SON; (2) maximal number of Fos-AVP (79 cells) and Fos-OXY (50 cells) double-labelings in the middle portion of the PVN; (3) low number of Fos-TH perikarya in the PVN and their lack in the SON; (4) similar incidence (around 50%) of Fos-AVP and Fos-OXY perikarya in the SON; and (5) presence of activated AVP, OXY, and TH neurons in the periventricular, subependymal, and sub-PVN zones of the PVN. Topographic analysis revealed that the majority of AVP neurons expressing Fos occupied the dorsolateral and central part of the middle portion of the PVN. In the same PVN portion, Fos-OXY neurons occurred in similar frequency, however, they were primarily distributed along the lateral and medial margins of the PVN. In the SON, Fos-OXY cells occupied mainly its dorsal, while Fos-AVP cells predominated in its ventral part. The data clearly indicate that HS is not a selective stimulus neither for PVN nor SON itself and provide evidence that both PVN and SON AVP and OXY cells play important role in the mediation of signals induced by HS. In addition, the limited number of AVP, OXY, and TH neurons activated by HS may account for their differential functional specializations selective for stress/osmotic circuits activated by HS. (C) 2004 Elsevier Ltd. All rights reserved.