Rapid detection and quantitation of human cytomegalovirus in urine through DNA hybridization.
Rapid detection and quantitation of human cytomegalovirus in urine through DNA hybridization.
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通过 DNA 杂交快速检测和定量尿液中的人巨细胞病毒。
DOI：
10.1056/nejm198304213081603
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Merigan,TC
中科院分区：
文献类型：
--
作者：
Chou,S;Merigan,TC
我们检测临床尿液标本中的巨细胞病毒DNA固定在硝酸纤维素过滤器和杂交的放射性标记，克隆片段的巨细胞病毒DNA。我们在24小时内完成了对9例巨细胞病毒尿症患者的39份尿液标本中病毒DNA的特异性检测和定量，大多数组织培养感染滴度为103/ml或更高。21例巨细胞病毒培养阴性患者的57份尿液标本均未出现假阳性结果。对巨细胞病毒尿症患者标本的分析表明，感染滴度与DNA杂交强度相关（r = 0.77）。从接受干扰素治疗的巨细胞病毒视网膜炎患者的连续尿液标本的杂交显示，在一段时间内，与临床结果相关的杂交病毒DNA的定量变化。该检测可用于选择患者进行抗病毒治疗和评估其疗效。（N Engl J Med 1983; 308：921-5.）
We detected cytomegalovirus DNA in clinical urine specimens after immobilization on nitrocellulose filters and hybridization with a radioactively labeled, cloned fragment of cytomegalovirus DNA. We accomplished the specific detection and quantitation of viral DNA within 24 hours with 39 urine specimens from nine patients with cytomegalovirus viruria, mostly at a tissue-culture infective titer of 103per milliliter or higher. None of 57 urine specimens from 21 patients that were culture-negative for cytomegalovirus gave false-positive results. Analysis of specimens from patients with cytomegalovirus viruria showed a correlation of the infective titer with the intensity of DNA hybridization (r = 0.77). Hybridization of sequential urine specimens from a patient undergoing treatment with interferon for cytomegalovirus retinitis revealed quantitative variations in hybridizable viral DNA over a period that correlated with clinical findings. This assay can be useful in the selection of patients for antiviral therapy and for the assessment of its efficacy. (N Engl J Med 1983; 308: 921–5.)