CONFORMATIONAL CHANGE IN PLASMA-ALBUMIN DUE TO INTERACTION WITH ISOLATED RAT HEPATOCYTE
CONFORMATIONAL CHANGE IN PLASMA-ALBUMIN DUE TO INTERACTION WITH ISOLATED RAT HEPATOCYTE
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DOI：
10.1152/ajpgi.1988.254.4.g465
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发表时间：
1988-04-01
影响因子：
--
通讯作者：
AWAZU, S
中科院分区：
文献类型：
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作者：
HORIE, T;MIZUMA, T;AWAZU, S
标记为牛血清白蛋白（BSA）的4-异硫氰酸酯-tempo的电子自旋共振光谱和标记为BSA的伊红马来酰亚胺在分离的大鼠肝细胞悬液中的吸收光谱表明白蛋白分子在与肝细胞膜相互作用时发生构象变化。白蛋白分子的构象变化可能加速了肝细胞表面白蛋白-有机阴离子复合物的解离。白蛋白分子的构象变化可以部分解释白蛋白介导的肝转运机制。
The electron spin resonance spectroscopy of 4-isothiocyanato-tempo labeled to bovine serum albumin (BSA) and the absorption spectroscopy of eosin maleimide labeled to BSA in the isolated rat hepatocyte suspension indicate conformational change occurring in the albumin molecule during interaction with the hepatocellular membrane. The conformational change in the albumin molecule may possibly accelerate the dissociation of albumin-organic anion complexes at the surface of the liver cell. The conformational change in the albumin molecule may explain in part the mechanism of albumin-mediated hepatic transport.