Effect of amino acid on calcium phosphate phase transformation: attenuated total reflectance-infrared spectroscopy and chemometrics
Effect of amino acid on calcium phosphate phase transformation: attenuated total reflectance-infrared spectroscopy and chemometrics
复制标题
氨基酸对磷酸钙相变的影响：衰减全反射红外光谱和化学计量学
DOI：
10.1007/s00396-018-4438-0
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发表时间：
2019
影响因子：
2.4
通讯作者：
Tanaka Hideji
中科院分区：
文献类型：
--
作者：
Otsuka Yuta;Ito Akira;Takeuchi Masaki;Tanaka Hideji
通过使用衰减全反射（ATR）-红外（IR）光谱研究了氨基酸对磷酸钙相变的影响。由磷酸四钙和磷酸二钙组成的自凝磷灰石水泥通过与磷酸溶液捏合转化为羟基磷灰石（HAp）。使用磷酸盐缓冲液（0.2 M，pH 6.8）或 0.1w/v% 氨基酸（丙氨酸、天冬酰胺、丝氨酸、ando-磷酸-l-丝氨酸）的水溶液作为捏合溶液。通过ATR-IR光谱分析捏合的样品。产生的 HAp 量通过基于 ATR-IR 光谱的多元曲线分辨率替代最小二乘法测定。 HAp 的相变适合多个固态动力学模型。结果表明，磷酸-L-丝氨酸抑制HAp晶体生长。利用分子动力学方法建立了HAp表面相互作用的模拟模型。 HAp 表面与氨基酸的总能量足以解释 theo-磷酸-l-丝氨酸抑制模型。这种抑制归因于 HAp 晶种表面对 ofo-磷酸-L-丝氨酸的特异性吸附。
The effects of amino acids on calcium phosphate phase transformation were investigated by using attenuated total reflection (ATR)-infrared (IR) spectroscopy. A self-setting apatite cement, which consisted of tetracalcium phosphate and dicalcium phosphate, was transformed into hydroxyapatite (HAp) by kneading with a phosphoric acid solution. Phosphate buffer (0.2 M, pH 6.8) or aqueous solutions of 0.1w/v% amino acids (alanine, asparagine, serine, ando-phospho-l-serine) were used as the kneading solutions. The kneaded samples were analyzed by ATR-IR spectroscopy. The amount of HAp produced was determined by the multivariate curve resolution alternative least-squares method based on the ATR-IR spectra. The phase transformations to HAp were fitted to several solid-state kinetic models. The results indicate thato-phospho-l-serine inhibits the crystal growth of HAp. A simulation model of HAp surface interaction was developed by using the molecular dynamics method. The total energy of the HAp surface with amino acids was sufficient to explain theo-phospho-l-serine inhibition model. The inhibition is attributed to the specific adsorption ofo-phospho-l-serine on the surface of HAp seed crystals.