A nanoemulsion of an anti-oxidant synergy formulation reduces tumor growth rate in neuroblastoma-bearing nude mice.
A nanoemulsion of an anti-oxidant synergy formulation reduces tumor growth rate in neuroblastoma-bearing nude mice.
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DOI：
10.1097/01.jnen.0000268844.82358.51
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发表时间：
2007-05
影响因子：
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通讯作者：
Fonghsu Kuo;Timothy Kotyla;T. Wilson;Lydia Kifle;T. Panagiotou;I. Gruverman;J. Tagne;Thomas Shea;R. Nicolosi
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文献类型：
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作者：
Fonghsu Kuo;Timothy Kotyla;T. Wilson;Lydia Kifle;T. Panagiotou;I. Gruverman;J. Tagne;Thomas Shea;R. Nicolosi
神经母细胞瘤是儿童癌症最常见的形式，可能由细胞分化的生化障碍和由此导致的增殖状态的持续而引起。神经母细胞瘤通常通过部分分化和最终变性自发恢复，并且可能与活性氧（ROS）的产生有关。我们最近在神经母细胞瘤细胞培养研究中报道，抗氧化协同制剂（ASF）可以诱导分化并缓冲培养的皮层神经元和载脂蛋白E缺陷小鼠的中枢神经系统组织中的神经元变性和氧化应激。本研究的目的是研究皮下注射和/或透皮应用 ASF 纳米乳制剂是否会降低神经母细胞瘤异种小鼠模型中的肿瘤生长速率。结果表明，尽管 ASF 悬浮液无法有效降低神经母细胞瘤小鼠模型中的肿瘤生长速率，但通过皮下注射或透皮应用 ASF 纳米乳制剂至肿瘤，肿瘤生长速率同样可平均降低 65%。总之，数据表明，皮下和/或透皮应用 ASF 纳米乳剂制剂可有效降低神经母细胞瘤小鼠模型中的肿瘤生长速度。
Neuroblastoma, the most common form of childhood cancer, may arise from a biochemical block of cellular differentiation and a resultant continuation of a proliferative state. Neuroblastoma often spontaneously reverts by undergoing partial differentiation and ultimate degeneration and may be associated with the generation of reactive oxygen species (ROS). We have recently reported in neuroblastoma cell culture studies that an anti-oxidant synergy formulation (ASF) can induce differentiation and buffer neuronal degeneration and oxidative stress in cultured cortical neurons and in central nervous system tissue of apolipoprotein E-deficient mice. The objective of the present study was to investigate whether a subcutaneous injection and/or transdermal application of a nanoemulsion preparation of ASF would reduce tumor growth rate in a neuroblastoma xenograph mouse model. The results indicate that whereas suspensions of ASF were ineffective in decreasing tumor growth rate in the neuroblastoma mouse model, tumor growth rate was similarly reduced an average 65% by either subcutaneous injection or transdermal application of an ASF nanoemulsion preparation to the tumor. In conclusion, the data suggest that subcutaneous and/or transdermal application of an ASF nanoemulsion preparation is effective in reducing tumor growth rate in this neuroblastoma mouse model.