Brassinosteroid-mediated apoplastic H2 O2 -glutaredoxin 12/14 cascade regulates antioxidant capacity in response to chilling in tomato.
Brassinosteroid-mediated apoplastic H2 O2 -glutaredoxin 12/14 cascade regulates antioxidant capacity in response to chilling in tomato.
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DOI：
10.1111/pce.13052
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发表时间：
2018-05
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影响因子：
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通讯作者：
X. Xia;Ping-Ping Fang-Ping;Xie Guo;Xiang-Jie Qian;Jie Zhou;K. Shi;Yanhong Zhou;Jingquan Yu
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作者：
X. Xia;Ping-Ping Fang-Ping;Xie Guo;Xiang-Jie Qian;Jie Zhou;K. Shi;Yanhong Zhou;Jingquan Yu
类维生素B1（BRs）调节植物的生长发育和胁迫反应。虽然已经了解了很多关于它们在植物发育中的作用，但BR调节植物胁迫耐受性的机制仍然不清楚。低温是影响植物生长的主要逆境。在这里，我们报告，BR积极调节番茄耐冷性。BR部分缺乏加剧了低温诱导的氧化蛋白积累、膜脂过氧化和光系统II最大量子效率（Fv/Fm）的降低。与此相反，BR生物合成基因Dwarf的过表达或24-表油菜素（EBR）处理减弱了低温诱导的氧化损伤，并导致Fv/Fm的增加。BR增加的转录本的爆裂氧化酶同源1（RBOH 1）和GRX基因，BR诱导的耐冷性与还原/氧化的2-半胱氨酸过氧化物酶（2-Cys Prx）的比例增加和抗氧化酶的激活。然而，RBOH 1-RNAi植物未能响应EBR方面的GRX基因的诱导，抗氧化能力的激活，和低温诱导的氧化损伤的衰减。此外，GRXS 12和S14的沉默损害了EBR诱导的还原/氧化2-Cys Prx和抗氧化酶活性的增加。我们的研究表明，BR通过涉及RBOH 1，GRXs和2-Cys Prx的信号级联增强番茄的耐冷性。
Brassinosteroids (BRs) regulate plant development and stress response. Although much has been learned about their roles in plant development, the mechanisms by which BRs regulate plant stress tolerance remain unclear. Chilling is a major stress that adversely affects plant growth. Here, we report that BR positively regulates chilling tolerance in tomato. BR partial deficiency aggravated chilling-induced oxidized protein accumulation, membrane lipid peroxidation, and decrease of maximum quantum efficiency of photosystem II (Fv/Fm). By contrast, overexpression of BR biosynthetic gene Dwarf or treatment with 24-epibrassinolide (EBR) attenuated chilling-induced oxidative damages and resulted in an increase of Fv/Fm. BR increased transcripts of RESPIRATORY BURST OXIDASE HOMOLOG1 (RBOH1) and GLUTAREDOXIN (GRX) genes, and BR-induced chilling tolerance was associated with an increase in the ratio of reduced/oxidized 2-cysteine peroxiredoxin (2-Cys Prx) and activation of antioxidant enzymes. However, RBOH1-RNAi plants failed to respond to EBR as regards to the induction of GRX genes, activation of antioxidant capacity, and attenuation of chilling-induced oxidative damages. Furthermore, silencing of GRXS12 and S14 compromised EBR-induced increases in the ratio of reduced/oxidized 2-Cys Prx and activities of antioxidant enzymes. Our study suggests that BR enhances chilling tolerance through a signalling cascade involving RBOH1, GRXs, and 2-Cys Prx in tomato.