Monkey embryonic stem cells differentiate into adipocytes in vitro
Monkey embryonic stem cells differentiate into adipocytes in vitro
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DOI：
10.1089/clo.2006.8.3
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发表时间：
2006-03-01
期刊：
影响因子：
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通讯作者：
Torii, R
中科院分区：
文献类型：
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作者：
Yamashita, A;Takada, T;Torii, R
功能性脂肪细胞的生产在脂肪细胞研究和再生医学中具有重要意义。在本文中，我们描述了猴胚胎干细胞（ES）分化为胰岛素反应性脂肪细胞。用成脂激素处理胚状体（EB）生长诱导脂肪细胞特异性基因的表达，如PPAR γ、C/EBP α、aP 2、胰岛素受体和GLUT 4。同时检测脂肪细胞因子瘦素和脂联素的表达。此外，在分化的脂肪细胞中通过胰岛素刺激观察到GLUT 4易位。这些结果表明，猴ES细胞可以作为研究灵长类动物脂肪形成的有用工具。
Production of functional adipocytes is important in adipocyte research and regenerative medicine. In this paper, we describe the differentiation of monkey embryonic stem (ES) cells into insulin-responsive adipocytes. Treatment of embryoid body (EB) outgrowth with adipogenic hormones induced the expression of adipocyte-specific genes, such as PPAR gamma, C/EBP alpha, aP2, insulin receptor, and GLUT4. Expression of adipocytokines, leptin and adiponectin, was also detected. Furthermore, translocation of GLUT4 was observed by insulin stimulation in differentiated adipocytes. These results suggested that monkey ES cells can be a useful tool for studying adipogenesis in primate.