Isolation and characterization of a novel pyrene-degrading Bacillus vallismortis strain JY3A.
Isolation and characterization of a novel pyrene-degrading Bacillus vallismortis strain JY3A.
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DOI：
10.1016/j.scitotenv.2011.02.020
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发表时间：
2011-04
期刊：
影响因子：
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通讯作者：
J. Ling;Guoying Zhang;H. Sun;Yuanyuan Fan;J. Ju;Changkai Zhang
中科院分区：
文献类型：
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作者：
J. Ling;Guoying Zhang;H. Sun;Yuanyuan Fan;J. Ju;Changkai Zhang
从山东中国济南炼油厂污染的土壤中新分离到一株多环芳烃降解菌JY3A。根据其16S rDNA序列、DNA-DNA亲缘关系和脂肪酸组成，以及各种生理特性，该菌株被鉴定为苦味芽孢杆菌。该菌株革兰氏阳性，游动，内孢子形成，需氧，氧化酶和过氧化氢酶阳性。细胞宽0.8~1.0μm，长2.0~2.5μm，呈单细胞或成对，有时呈链状。苦味芽孢杆菌JY3A菌株能以萘、菲、芘、荧烯、苯、甲苯、苯酚、甲醇、乙醇、吐温80、环己烷或邻苯二酚为唯一碳源。在0.5%(w/w)吐温80存在下，该菌株单独在初始浓度为150ppm的情况下，15天内就能去除90.5%的芘。而JY3A与黄孢原毛平革菌共培养7d后，可使芘的浓度降低近55.4%。
The PAHs-degrading bacterium strain JY3A was newly isolated from the polluted soil in the Jinan Oil Refinery Factory, Shandong Province of China. The isolate was identified as Bacillus vallismortis with respect to its 16S rDNA sequence, DNA–DNA relatedness and fatty acid profiles, as well as various physiological characteristics. The strain was Gram-positive, motile, endospore forming, aerobic, oxidase and catalase-positive. The cells were 0.8–1.0μm wide and 2.0–2.5μm long, single or in pairs and sometimes in chains. Bacillus vallismortis strain JY3A could utilize naphthalene, phenanthrene, anthracene, pyrene, fluorene, benzene, toluene, phenol, methanol, ethanol, Tween 80, cyclohexane or catechol as sole carbon source. The strain alone removed 90.5% of pyrene at an initial concentration of 150ppm in 15days in the presence of 0.5% (w/w) Tween 80. However, in co-culture with Phanerochaete chrysosporium, JY3A reduced the concentration of pyrene by nearly 55.4% after 7days of incubation.