The secreted EGF-Discoidin factor xDel1 is essential for dorsal development of the Xenopus embryo
The secreted EGF-Discoidin factor xDel1 is essential for dorsal development of the Xenopus embryo
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DOI：
10.1016/j.ydbio.2007.03.008
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发表时间：
2007-06-01
影响因子：
2.7
通讯作者：
Sasai, Yoshiki
中科院分区：
文献类型：
--
作者：
Arakawa, Akiko;Matsuo-Takasaki, Mami;Sasai, Yoshiki
我们在这里表明，分泌的egf -盘状蛋白结构域蛋白Xenopus Del1 （xDel1）是早期爪蟾胚胎中背部发育的重要因素。敲除xDel1功能会导致胚胎明显的腹化。相反，在原胚中，过表达xDel1会扩大背侧标记的表达，抑制腹侧标记的表达。强迫表达xDel1能促进腹缘区外植体的分化，而微弱地诱导神经分化，但不能诱导中胚层分化。xDel1的活化活性依赖于盘状蛋白结构域，而不依赖于RGD基序（与血管生成活性有关）或EGF重复序列。荧光素酶分析表明，xDel1通过干扰BMP受体下游通路，减弱BMP信号报告蛋白的活性。因此，xDel1在早期脊椎动物胚胎发生中作为DV模式的独特细胞外调节因子发挥作用。(C) 2007爱思唯尔公司版权所有。
We show here that a secreted EGF-Discoidin-domain protein, Xenopus Del1 (xDel1), is an essential factor for dorsal development in the early Xenopus embryo. Knockdown of the xDel1 function causes obvious ventralization of the embryo. Conversely, overexpression of xDel1 expands dorsal-marker expression and suppresses ventral-marker expression in the gastrula embryo. Forced expression of xDel1 dorsalizes ventral marginal zone explants, whereas it weakly induces neural differentiation but not mesodermal differentiation in animal caps. The dorsalizing activity of xDel1 is dependent on the Discoidin domains and not on the RGD motif (which is implicated in its angiogenic activity) or EGF repeats. Luciferase assays show that xDel1 attenuates BMP-signaling reporter activity by interfering with the pathway downstream of the BMP receptor. Thus, xDel1 functions as a unique extracellular regulatory factor of DV patterning in early vertebrate embryogenesis. (C) 2007 Elsevier Inc. All rights reserved.