Comparison of rates of virus isolation from leukocyte populations separated from blood by conventional and Ficoll-Paque/Macrodex methods
Comparison of rates of virus isolation from leukocyte populations separated from blood by conventional and Ficoll-Paque/Macrodex methods
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通过传统方法和 Ficoll-Paque/Macrodex 方法从血液中分离的白细胞群中分离病毒的率的比较
DOI：
10.1128/jcm.10.4.533-537.1979
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发表时间：
1979
影响因子：
9.4
通讯作者：
W. J. Martin
中科院分区：
文献类型：
--
作者：
C. L. Howell;M. Miller;W. J. Martin
将152份血液标本（主要来自免疫功能低下的患者）采集到肝素化采血管中，并分成等体积的成对等分试样。通过常规和Ficoll-Paque/Macrodex（F-P/M）方法处理血液，获得含有混合白细胞和单独单核细胞和多形核白细胞群的血沉棕黄层制剂。采用两种方法获得的细胞悬液接种WI-38成纤维细胞单层，观察细胞病变效应的发展，以评估病毒感染性。使用F-P/M获得了两倍多的病毒分离。在常规和F-P/M分离的病毒中，后者的细胞病变效应更广泛。此外，与传统方法相比，使用F-P/M方法分离出的病毒种类更多。F-P/M方法并不比常规方法更耗时，易于适用于诊断病毒学实验室，仅需要最小的额外成本，并且是监测病毒血症的特别合适和有效的手段。
One hundred fifty-two blood specimens, largely from immunocompromised patients, were collected in heparinized Vacutainer tubes and divided into paired aliquots of equal volume. Buffy-coat preparations, containing mixed leukocyte and separate mononuclear and polymorphonuclear leukocyte populations were obtained by treatment of blood with conventional and Ficoll-Paque/Macrodex (F-P/M) methods. The development of cytopathic effect in monolayers of WI-38 fibroblasts inoculated with cell suspensions derived from the two methods was used to assess virus infectivity. Twice as many virus isolations were obtained using F-P/M. Of those viruses isolated by both conventional and F-P/M, the development of cytopathic effect was more extensive using the latter method. Moreover, a greater variety of viruses was isolated using F-P/M method, as compared to the conventional method. The F-P/M method is no more time consuming than conventional procedures, is readily adaptable for use in the diagnostic virology laboratory, requires only minimal additional cost, and is a particularly suitable and effective means of monitoring viremia.