Accumulation of selenium in mixotrophic culture of Spirulina platensis on glucose
Accumulation of selenium in mixotrophic culture of Spirulina platensis on glucose
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DOI：
10.1016/j.biortech.2005.10.038
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发表时间：
2006-12-01
影响因子：
11.4
通讯作者：
Bai, Yan
中科院分区：
文献类型：
--
作者：
Chen, Tianfeng;Zheng, Wenjie;Bai, Yan
以螺旋藻为研究对象，研究了混合营养培养对硒积累的影响。结果表明，葡萄糖作为混合营养培养的有机碳源优于乙酸。添加葡萄糖（2 g L-1）显著提高了白颡鱼生物量（2.57 g L-1）、藻蓝蛋白（0.279 g L-1）和异藻蓝蛋白（0.126 g L-1）的产量，显著高于光自养培养（1.08 g L-1、0.119 g L-1和0.042 g L-1）。在生长阶段逐步添加硒，避免了高浓度硒对银杏生长的抑制作用。硒的富集有利于藻蓝蛋白和异藻蓝蛋白的生成。硒累积浓度为250 mg L-1时，硒产量最高（1033 μ g L-1），有机硒含量、生物量、藻蓝蛋白和异藻蓝蛋白产量分别为92.3%、2.55 g L-1、0.295 g L-1和0.153 g L-1。上述结果表明，在混合营养培养基中逐步添加硒，可为生产高富硒藻产品提供一条经济有效的途径。(c) 2005 Elsevier Ltd版权所有。
Accumulation of Se in mixotrophic culture of Spirulina platensis was investigated in this study. Results indicated that glucose was better than acetate as an organic carbon source for mixotrophic culture of S. platensis. Supplementation of glucose (2 g L-1) significantly enhanced the biomass concentration (2.57 g L-1) and the production of phycocyanin (0.279 g L-1) and allophycocyanin (0.126 g L-1) in S. platensis, which were much higher than those of photoautotrophic culture (1.08 g L-1, 0.119 g L-1 and 0.042 g L-1, respectively). Stepwise addition of Se during the growth phase avoided the inhibitory effect of high Se concentration on the growth of S. platensis. The Se enrichment favored the production of phycocyanin and allophycocyanin in the algal cells. The highest Se yield (1033 mu g L-1) was obtained at an accumulative Se concentration of 250 mg L-1, with organic Se percentage, biomass concentration, phycocyanin and allophycocyanin yields of 92.3%, 2.55 g L-1, 0.295 g L-1 and 0.153 g L-1, respectively. These results indicated that the application of mixotrophic culture S. platensis with stepwise addition of Se to the medium could offer an effective and economical way for the production of high Se-enriched algal products. (c) 2005 Elsevier Ltd. All rights reserved.