Characterization of ScORK28, a transmembrane functional protein receptor kinase predominantly expressed in ovaries from the wild potato species Solanum chacoense
Characterization of ScORK28, a transmembrane functional protein receptor kinase predominantly expressed in ovaries from the wild potato species Solanum chacoense
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DOI：
10.1016/j.febslet.2007.10.001
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发表时间：
2007-10
期刊：
影响因子：
3.5
通讯作者：
H. Germain;J. Houde;M. Gray-Mitsumune;T. Sawasaki;Y. Endo;J. Rivoal;D. P. Matton
中科院分区：
文献类型：
--
作者：
H. Germain;J. Houde;M. Gray-Mitsumune;T. Sawasaki;Y. Endo;J. Rivoal;D. P. Matton
从一个富含弱表达mrna的胚珠cDNA文库中发现了30个受体激酶，其中发现了茄珠受体激酶28 （ScORK28）。该LRR-RLK在RNA和蛋白质水平上表现出高水平的组织特异性，主要表达于雌性生殖组织中。在植物中的蛋白表达分析显示，ScORK28被n -糖基化，ScORK28::GFP融合分析显示其定位在质膜上。细菌对ScORK28催化结构域的表达和激酶活性分析表明，ScORK28是一种活性的Mg2+依赖性蛋白激酶，并且近膜结构域是激酶活性所必需的。
Solanum chacoense ovule receptor kinase 28 (ScORK28) was found among 30 receptor kinases from an ovule cDNA library enriched for weakly expressed mRNAs. This LRR-RLK displayed high level of tissue specificity at the RNA and protein levels and was predominantly expressed in female reproductive tissues. Protein expression analyses in planta revealed that ScORK28 was N-glycosylated and ScORK28::GFP fusion analyses showed that it was localized at the plasma membrane. Bacterial expression of ScORK28 catalytic domain followed by kinase activity assays revealed that ScORK28 is an active Mg2+-dependent protein kinase and that the juxtamembrane domain is necessary for kinase activity.