Long-term multiple color imaging of live cells using quantum dot bioconjugates
Long-term multiple color imaging of live cells using quantum dot bioconjugates
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DOI：
10.1038/nbt767
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发表时间：
2003-01-01
影响因子：
46.9
通讯作者：
Simon, SM
中科院分区：
文献类型：
--
作者：
Jaiswal, JK;Mattoussi, H;Simon, SM
发光量子点（QD）-半导体纳米材料-是一种很有前途的替代有机染料的荧光应用。我们已经开发出使用量子点标记活细胞的程序，并已证明它们可用于活细胞的长期多色成像。提出的两种方法是（i）QD的内吞摄取和（ii）用缀合至抗体的QD选择性标记细胞表面蛋白。使用这些方法标记的活细胞用于长期荧光成像。随着细胞的生长和发育，细胞保持稳定标记超过一周。这些方法应该允许在长时间内同时研究多个细胞，因为它们通过生长和发育进行。
Luminescent quantum dots (QDs)-semiconductor nanocrystals-are a promising alternative to organic dyes for fluorescence-based applications. We have developed procedures for using QDs to label live cells and have demonstrated their use for long-term multicolor imaging of live cells. The two approaches presented are (i) endocytic uptake of QDs and (ii) selective labeling of cell surface proteins with QDs conjugated to antibodies. Live cells labeled using these approaches were used for long-term multicolor imaging. The cells remained stably labeled for over a week as they grew and developed. These approaches should permit the simultaneous study of multiple cells over long periods of time as they proceed through growth and development.