Serological study of the 2009 pandemic due to influenza A H1N1 in the metropolitan French population
Serological study of the 2009 pandemic due to influenza A H1N1 in the metropolitan French population
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DOI：
10.1111/j.1469-0691.2011.03545.x
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发表时间：
2012-02-01
影响因子：
14.2
通讯作者：
de Lamballerie, X.
中科院分区：
文献类型：
--
作者：
Delangue, J.;Salez, N.;de Lamballerie, X.
我们使用标准血凝抑制和微量中和试验，在法国大城市居民的血清中寻找2009年甲型H1N1流感大流行病毒抗体的证据，这些血清是在疫情发生之前、期间或之后获得的。在流行后和流行前面板中测量的血清阳性率之间的差异被用来估计不同年龄组的血清转化率(23.4%（024岁，0年龄组）；16.5% (2534);7.9% (3544);7.2% (4554);1.6% (5564);3.1% (bbb65))，证实不同年龄组的病例分布与季节性H1N1病毒相似。在大流行前期间，大部分人群中存在低滴度的交叉反应性抗体（可能是针对季节性H1N1获得的），而在65岁以上的个体中检测到交叉反应性抗体，其患病率和血清学滴度明显较高（可能是先前针对西班牙流感相关H1N1毒株获得的）。临床数据和大流行后血清流行率分析显示，这些患者中很少有人在大流行期间感染流感病毒。相比之下，大多数临床病例和血清转化都记录在24岁年龄组，并且在大流行前时期，在各年龄组中观察到pH1N1抗体流行率与血清转化率之间的全球反比关系。我们的研究结果强调了流感大流行和季节性H1N1病毒抗原抗原反应的复杂关系；因此，在区分低滴度特异性抗体和交叉反应性抗体、建立精确的血清阳性率数字和充分理解先前对季节性病毒的免疫与对新变种的保护之间的关系方面存在困难。
We looked for evidence of antibodies to the 2009 influenza A/H1N1 pandemic virus in panels of sera from individuals living in metropolitan France, obtained either before, during or after the epidemic, using standard haemagglutination inhibition and microneutralization tests. The difference between seroprevalence values measured in post- and pre-epidemic panels was used as an estimate of seroconversion rate in different age groups (23.4% (024 years, age-group 0); 16.5% (2534); 7.9% (3544); 7.2% (4554); 1.6% (5564); and 3.1% (>65)), confirming that the distribution of cases in different age groups was similar to that of the seasonal H1N1 virus. During the pre-pandemic period low-titre cross-reactive antibodies were present in a large proportion of the population (presumably acquired against seasonal H1N1) whereas cross-reactive antibodies were detected in individuals over the age of 65 years with significantly higher prevalence and serological titres (presumably acquired previously against Spanish flu-related H1N1 strains). Clinical data and analysis of post-pandemic seroprevalence showed that few of these latter patients were infected by the influenza virus during the epidemic. In contrast, the majority of both clinical cases and seroconversions were recorded in the 024 age group and a global inverse relationship between prevalence of antibodies to pH1N1 in the pre-pandemic period and rate of seroconversion was observed amongst age groups. Our results emphasize the complex relationships involved in antigenic reactivity to pandemic and seasonal H1N1 viral antigens; hence the difficulty in distinguishing between low-titre specific and cross-reactive antibodies, establishing precise seroprevalence numbers and fully understanding the relationship between previous immunity to seasonal viruses and protection against the novel variant.