Xpat, a gene expressed specifically in germ plasm and primordial germ cells of Xenopus laevis
Xpat, a gene expressed specifically in germ plasm and primordial germ cells of Xenopus laevis
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DOI：
10.1016/s0925-4773(98)00047-1
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发表时间：
1998-05-01
影响因子：
2.6
通讯作者：
Woodland, HR
中科院分区：
文献类型：
--
作者：
Hudson, C;Woodland, HR
以非洲爪蟾原胚植物极特异性cDNA为探针，筛选原胚植物极cDNA文库。其中一个新克隆的RNA表达模式与其种质成分一致，因此被命名为Xpat （Xenopus primordial germ cell associated transcript）。开放阅读框编码一个35 kDa的蛋白，没有明确的同源性。RNA在整个卵发生和卵裂早期都定位于植物极。在原肠形成过程中，含有这种信息的细胞在内部移动，在尾芽阶段，它们向前后方向移动。一旦背肠系膜形成，Xpat mRNA就无法检测到。我们发现3'-UTR是必需的，并且足以将外源RNA定位到卵母细胞的植物极。我们认为含有Xpat utr的转录本可以在卵子发生的第三阶段通过Vgl或mRNA定位的晚期途径定位，但内源性Xpat似乎通过类似于Xcat-2的线粒体云机制更早地定位。(C) 1998爱思唯尔科学爱尔兰有限公司版权所有。
cDNAs specific to vegetal poles of Xenopus gastrula embryos were used as a probe to screen a gastrula vegetal pole cDNA library. One of the novel clones isolated had an RNA expression pattern consistent with it being a component of germ plasm and it was thus named Xpat (Xenopus primordial germ cell associated transcript). The open reading frame encodes a 35 kDa protein with no clear homologies. The RNA is localised to the vegetal pole throughout oogenesis and early cleavage. During gastrulation cells containing this message move internally and at tailbud stages they migrate in an antero-dorsal direction. Xpat mRNA is not detectable once the dorsal mesentery forms. We show that the 3'-UTR is required and is sufficient for localisation of exogenous RNA to the vegetal pole of oocytes. We propose that Xpat UTR-containing transcripts can be localised by the Vgl or late pathway of mRNA localisation during stage III of oogenesis, but endogenous Xpat appears to be localised earlier by a mitochondrial cloud mechanism similar to that proposed for Xcat-2. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.