Isolation and co-cultivation of human macrophages and neutrophils with Plasmodium falciparum-parasitized erythrocytes: An optimized system to study the phagocytic activity to malarial parasites
Isolation and co-cultivation of human macrophages and neutrophils with Plasmodium falciparum-parasitized erythrocytes: An optimized system to study the phagocytic activity to malarial parasites
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人类巨噬细胞和中性粒细胞与恶性疟原虫寄生红细胞的分离和共培养：研究疟疾寄生虫吞噬活性的优化系统
DOI：
10.1016/j.parint.2016.03.005
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发表时间：
2016
影响因子：
1.9
通讯作者：
Kato K.
中科院分区：
文献类型：
--
作者：
Terkawi MA;Takano;R;Kato K.
巨噬细胞和中性粒细胞是我们抵御入侵病原体的最前线。它们是专职吞噬细胞，在从宿主循环中清除恶性疟原虫寄生的红细胞中发挥关键作用。这些细胞和寄生红细胞的稳定体外培养系统将提供研究其对感染的免疫反应的手段，可能为疟疾的新治疗干预提供重要线索。在这里，我们提出了一个优化的方案，培养人外周血单核细胞衍生的巨噬细胞和中性粒细胞与恶性疟原虫寄生的红细胞。
Macrophages and neutrophils are our front line of defense against invading pathogens. They are professional phagocytic cells that play a key role in the clearance of Plasmodium falciparum-parasitized erythrocytes from the host circulation. A stablein vitroculture system for these cells and parasitized erythrocytes would provide the means to study their immunological responses to infection, potentially revealing important clues for new therapeutic interventions for malaria. Here, we present an optimized protocol for cultivating human peripheral blood monocyte-derived macrophages and neutrophils withPlasmodium falciparum-parasitized erythrocytes.