Determining S-genotypes of two sweet cherry (Prunus avium L.) cultivars, 'Takasago (Rockport Bigarreau)' and 'Hinode (Early Purple)'
Determining S-genotypes of two sweet cherry (Prunus avium L.) cultivars, 'Takasago (Rockport Bigarreau)' and 'Hinode (Early Purple)'
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确定两个甜樱桃 (Prunus avium L.) 品种“Takasago (Rockport Bigarreau)”和“Hinode (Early Purple)”的 S 基因型
DOI：
10.2503/jjshs.69.29
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发表时间：
2000
影响因子：
--
通讯作者：
A. Sugiura
中科院分区：
文献类型：
--
作者：
H. Yamane;R. Tao;H. Murayama;Makoto Ishiguro;Y. Abe;J. Soejima;A. Sugiura
日本甜樱桃主要早熟品种之一“高砂”与“日出”杂交不亲和。因此，他们被认为具有相同的S等位基因，尽管他们的S基因型未知。然而，当'高砂'与'日之'在山形县园艺实验站异花授粉时，结果正常（未发表的数据），这表明它们是交叉兼容的。在这项研究中，我们重新评估了两个品种之间的可交配性控制授粉试验，并确定其S-基因型的分子分型系统最近开发的甜樱桃S-等位基因。在日本两个不同地点进行的异花授粉试验表明，‘高砂’和‘日之’是自交不亲和的，但它们是异花亲和的。对花柱提取物的双向聚丙烯酰胺凝胶电泳（2D-PAGE）分析表明，它们不含甜樱桃花柱中常见的S基因产物S-RNases，表明它们的S基因型完全不同。根据2D-PAGE图谱中的S-RNase斑点图和限制性片段长度多态性（RFLP）分析，“高砂”和“日出”的S基因型分别为S1 S6和S2 Sx（Sx是不同于S1至S6的新的S等位基因）。这意味着'Hinode'与已知S基因型的所有品种杂交亲和。本研究中采用的S-等位基因分型的生物化学和分子方法的有用性进行了讨论。
'Takasago (Rockport Bigarreau)', one of the major early ripening cultivars of sweet cherry in Japan, was reported to be cross-incompatible with 'Hinode (Early Purple)'. Thus, they were assumed to have the same S-alleles, although their S-genotypes were unknown. However, when 'Takasago' was cross-pollinated with 'Hinode' at the Yamagata Prefectural Horticultural Experiment Station, the fruit set normally (unpublished data), which suggests that they are cross-compatible. In this study, we re-evaluated crossability between the two cultivars by controlled pollination tests and determined their S-genotypes by the molecular typing system recently developed for S-alleles of sweet cherry. The cross-pollination tests conducted at two different locations in Japan showed that 'Takasago' and 'Hinode' were self-incompatible but reciprocally cross-compatible. Furthermore, 2D-PAGE (two dimentional-polyacrylamide gel electrophoresis) analysis for stylar extracts revealed that they have none of the common S-RNases, S-gene products in styles of sweet cherry, which suggests that their S-genotypes are completely different. By S-RNase spot patterns in 2D-PAGE profiles and RFLP (restriction fragment length polymorphism) analysis, S-genotypes of 'Takasago' and 'Hinode' were proposed to be S1S6 and S2Sx(Sx is a novel S-allele different from S1 to S6), respectively. This implies that 'Hinode' is cross-compatible with all cultivars of known S-genotypes. The usefulness of biochemical and molecular methods for S-allele typing employed in this study is discussed.