A paper disk equipped with graphene/polyaniline/Au nanoparticles/glucose oxidase biocomposite modified screen-printed electrode: Toward whole blood glucose determination
A paper disk equipped with graphene/polyaniline/Au nanoparticles/glucose oxidase biocomposite modified screen-printed electrode: Toward whole blood glucose determination
复制标题
配备石墨烯/聚苯胺/金纳米粒子/葡萄糖氧化酶生物复合材料修饰丝网印刷电极的纸盘：迈向全血糖测定
DOI：
10.1016/j.bios.2013.12.067
复制
发表时间：
2014-06-15
影响因子：
12.6
通讯作者：
Wang, Wei
中科院分区：
文献类型：
--
作者：
Kong, Fen-Ying;Gu, Sai-Xi;Wang, Wei
本工作利用一次性丝网印刷碳电极(SPCE)与纸盘联用，建立了一种方便、快速、低成本、小样本量、原位检测人全血中葡萄糖的方法。为了进行分析，用石墨烯/聚苯胺/金纳米颗粒/葡萄糖氧化酶(Gr/PANI/AuNPs/GOD)生物复合材料修饰SPCE，然后用浸渍样品的纸盘覆盖。在纸盘上引入PbS后，进行了电化学测试。用差示脉冲伏安法(DPV)检测酶-底物反应引起的GOD中黄素腺嘌呤二核苷酸(FAD)电流的降低。分析性能与传统方法相当，涵盖了全血中临床相关的葡萄糖浓度的全部范围。这种新型纸基电化学葡萄糖传感器在全血检测中的应用前景看好，尤其适合在发展中国家和资源有限的环境中使用。(C)2014爱思唯尔B.V.保留所有权利。
In this work, a convenient, fast, low cost, small sample volume and in situ detection of glucose in human whole blood has been developed by using a disposable screen-printed carbon electrode (SPCE) coupled with a paper disk. To perform the assay, the SPCE was modified with graphene/polyaniline/Au nanoparticles/glucose oxidase (Gr/PANI/AuNPs/GOD) biocomposite and then covered by a paper disk impregnated with the sample. After introducing PBS on the paper disk, the electrochemical measurement was carried out. The assay was based on measuring the current decrease of flavin adenine dinucleotide (FAD) in GOD provoked by the enzyme-substrate reaction using differential pulse voltammetry (DPV). The analytical performance was comparable to conventional methods, and covered the full range of clinically relevant concentrations of glucose in whole blood. This new paper-based electrochemical glucose sensor shows promise in applying point-of-care (POC) device in whole blood tests, and particularly being appropriate for use in the developing world and in resource-limited settings. (C) 2014 Elsevier B.V. All rights reserved.