pHi controls cytoplasmic calcium in rat parotid cells.
pHi controls cytoplasmic calcium in rat parotid cells.
复制标题
pHi 控制大鼠腮腺细胞中的细胞质钙。
DOI：
10.1016/0005-2736(92)90019-i
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Cashman,JR
中科院分区：
文献类型：
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作者：
Snowdowne,KW;Way,B;Thomas,G;Chen,HY;Cashman,JR
本研究的目的是确定胞浆pH值（pHi）是否调制大鼠腮腺细胞胞浆中钙离子浓度（Cai）的基础水平。我们研究了各种实验操作对BCECF和钙光蛋白水母发光蛋白测定的pH和Caias的影响。我们发现，各种实验操作，增加pHi，如暴露的细胞NH4Cl，降低CO2分压或增加细胞外pH值的存在下，尼日利亚菌素总是增加蔡。此外，降低Cai的实验操作，如减少细胞外[NaHCO3]或去除负载的NH4总是降低Cai。因此，腮腺细胞中的pH和CaI直接相关。由于最近的研究表明，Cai直接影响pHi，我们认为，Cai处理和pHi处理在腮腺细胞中紧密相连。
The goal of this investigation was to determine if cytoplasmic pH (pHi) modulated the basal level of the concentration of calcium ions in the cytoplasm (Cai) in rat parotid cells. We investigated the effects of various experimental manipulations on both pHiand Caias measured with BCECF and the calcium photoprotein aequorin, respectively. We found that various experimental manipulations that increased pHi, such as exposure of the cells to NH4Cl, a decrease of the partial pressure of CO2or an increase in extracellular pH in the presence of nigericin invariably increased Cai. Moreover, experimental manipulations which lowered Cai, such as a reduction of extracellular [NaHCO3] or the removal of loaded NH4invariably decreased Cai. Thus pHiand Caiare directly related in parotid cells. Since recent studies have shown that Caidirectly influences pHi, we suggest that Cai-handling and pHi-handling are tightly linked in parotid cells.