Glycogen synthase kinase-3β is highly activated in nuclei and mitochondria
Glycogen synthase kinase-3β is highly activated in nuclei and mitochondria
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DOI：
10.1097/00001756-200312190-00025
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发表时间：
2003-12-19
期刊：
影响因子：
1.7
通讯作者：
Jope, RS
中科院分区：
文献类型：
--
作者：
Bijur, GN;Jope, RS
糖原合成酶激酶-3 β（GSK 3 β）主要位于细胞质中，但也存在于细胞核和线粒体中。在SH-SY 5 Y细胞、原代皮层神经元和小鼠脑中，细胞核和线粒体中活性GSK 3 β（丝氨酸-9上未磷酸化）的比例是胞质中的5- 8倍。相应地，细胞核和线粒体中的GSK 3 β活性高于细胞质。通过喜树碱或毒胡萝卜素处理的凋亡信号刺激激活细胞核和线粒体中的GSK 3 β，但不激活胞质溶胶中的GSK 3 β，而通过锂处理的GSK 3 β抑制影响所有三个GSK 3 β库。因此，细胞核和线粒体含有不成比例的高水平的活性GSK 3 β，其被一些凋亡刺激物选择性地进一步激活。(C)2003年利平科特威廉姆斯威尔金斯。
Glycogen synthase kinase-3beta (GSK3beta) is located predominantly in the cytosol, but also is in nuclei and mitochondria. In SH-SY5Y cells, primary cortical neurons, and mouse brain, the portion of active GSK3beta (not phosphorylated on serine-9) was 5- to 8-fold greater in nuclei and mitochondria than in cytosol. Correspondingly greater GSK3beta activities were measured in nuclei and mitochondria compared with cytosol. Stimulation of apoptotic signaling by treatment with camptothecin or thapsigargin activated GSK3beta in the nucleus and mitochondria, but not the cytosol, whereas inhibition of GSK3beta by lithium treatment affected all three pools of GSK3beta. Thus, the nuclei and mitochondria contain disproportionately high levels of active GSK3beta, which is selectively further activated by some apoptotic stimuli. (C) 2003 Lippincott Williams Wilkins.