Increased osteopontin levels in the vitreous of patients with diabetic retinopathy
Increased osteopontin levels in the vitreous of patients with diabetic retinopathy
复制标题
DOI：
10.1159/000102936
复制
发表时间：
2007-01-01
影响因子：
2.1
通讯作者：
Uede, Toshimitsu
中科院分区：
文献类型：
--
作者：
Kase, Satoru;Yokoi, Masahiko;Uede, Toshimitsu
目的：骨桥蛋白（OPN）具有细胞粘附、趋化、免疫调节和血管生成等多种功能。本研究的目的是分析糖尿病视网膜病变（DR）和非DR患者玻璃体液中OPN的水平。方法：11例DR患者中19例和8例非DR患者行玻璃体切割术，同时采集玻璃体液。应用两种不同的夹心酶联免疫吸附测定系统（系统1和2），其已经在我们的实验室中开发以定量玻璃体液中的OPN浓度。结果如下：使用系统1，玻璃体液中的非凝血酶切割的全长OPN水平在DR中为921.63 ± 45.38ng/ ml，在非DR中为632.80 ± 83.43ng/ ml.此外，在使用系统2的DR和非DR患者中，玻璃体凝血酶切割和非切割OPN水平增加至2，109.22 +/-151.651和1，651.13 +/-229.82 ng/ ml. DR中的玻璃体OPN水平显著高于非DR中的玻璃体OPN水平（系统1的p < 0.01，系统2的p < 0.05）。结论：DR患者凝血酶切割的和未切割的玻璃体OPN水平与对照受试者相比增加，表明OPN在糖尿病视网膜缺血的发病机制中起潜在作用。版权所有（c）2007 S. Karger AG，巴塞尔。
Purpose: Osteopontin ( OPN) has diverse functions such as cell adhesion, chemoattraction, immunomodulation, and angiogenesis. The aim of this study is to analyze the OPN levels in vitreous fluid obtained from diabetic retinopathy ( DR) and non- DR patients. Methods: Nineteen patients out of 11 with DR and 8 without DR underwent pars plana vitrectomy and vitreous fluid was obtained simultaneously. Two distinct sandwich enzyme- linked immunosorbent assay systems ( systems 1 and 2) were applied, which have been developed in our laboratories to quantify the OPN concentrations in vitreous fluid. Results: The non- thrombin- cleaved full- length OPN levels in the vitreous fluid were 921.63 8 45.38 ng/ ml in DR and 632.80 +/- 83.43 ng/ ml in non- DR using system 1. Also, vitreous thrombin- cleaved and noncleaved OPN levels were increased to 2,109.22 +/- 151.651 and 1,651.13 +/- 229.82 ng/ ml in patients with DR and non- DR using system 2. The vitreous OPN levels were significantly higher in DR than those in non- DR ( p < 0.01 by system 1 and p < 0.05 by system 2). Conclusion: Thrombin- cleaved and noncleaved vitreous OPN levels in patients with DR were increased compared with control subjects, suggesting that OPN plays a potential role in the pathogenesis of diabetic retinal ischemia. Copyright (c) 2007 S. Karger AG, Basel.