The ethylene response factor MdERF1B regulates anthocyanin and proanthocyanidin biosynthesis in apple
The ethylene response factor MdERF1B regulates anthocyanin and proanthocyanidin biosynthesis in apple
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乙烯反应因子MdERF1B调节苹果花青素和原花青素的生物合成
DOI：
10.1007/s11103-018-0770-5
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发表时间：
2018-10-01
影响因子：
5.1
通讯作者：
Chen, Xuesen
中科院分区：
文献类型：
--
作者：
Zhang, Jing;Xu, Haifeng;Chen, Xuesen
Key messageThe regulator MdERF1B in the apple (Malus×domestica) ethylene pathway mainly acts on MdMYB9 and MdMYB11 to regulate anthocyanin and proanthocyanidin accumulation.AbstractDietary anthocyanins and proanthocyanidins (PAs) have health benefits for humans, and are associated with decreased risks of coronary heart disease and cancer. Ethylene can enhance reddening of apple (Malus×domestica), but the regulatory mechanism is poorly understood. In this study, an ethylene response factor (ERF), MdERF1B, was identified and functionally characterized. ‘Orin’ calli overexpressingMdERF1Bwere generated and then analyzed by quantitative reverse transcription-PCR. Compared with the control calli, theMdERF1B-overexpressing calli showed increased expression levels ofMdACO1, MdERF1, andMdERF3in the ethylene pathway andMdCHS, MdCHI, MdF3H, MdDFR, MdANS, MdLAR, MdANR, MdMYB9andMdMYB11in the flavonoid pathway. As a result, the levels of anthocyanins and PAs were significantly increased in theMdERF1B-overexpressing calli. MdERF1B interacted with MdMYB9, MdMYB1, and MdMYB11 proteins in yeast two-hybrid, pull-down, and bimolecular fluorescence complementation assays. Furthermore, in yeast one-hybrid and electrophoretic mobility shift assays, MdERF1B also bound to the promoters ofMdMYB9, MdMYB1, andMdMYB11. In a luciferase reporter assay, MdERF1B mainly activatedproMdMYB9andproMdMYB11, promoting their expression levels. This was in agreement withMdERF1B’s overexpression in calli, which barely affectedMdMYB1expression. Taken together, our findings provide an insight into the regulatory mechanisms in the ethylene pathway that increase anthocyanin and PA accumulation in apple.
