Flow Cytometry to Assess the Counts and Physiological State of Cronobacter sakazakii Cells after Heat Exposure
Flow Cytometry to Assess the Counts and Physiological State of Cronobacter sakazakii Cells after Heat Exposure
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DOI：
10.3390/foods8120688
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发表时间：
2019-06
期刊：
影响因子：
5.2
通讯作者：
P. Cal-Sabater;I. Caro;M. J. Castro;María-José Cao;J. Mateo;E. J. Quinto
中科院分区：
文献类型：
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作者：
P. Cal-Sabater;I. Caro;M. J. Castro;María-José Cao;J. Mateo;E. J. Quinto
坂崎克罗伊氏菌是一种机会致病菌，与新生儿坏死性小肠结肠炎、败血症和脑膜炎的暴发有关。婴儿配方奶粉是最常见的传染媒介。本研究的目的是了解C.使用流式细胞术检测坂崎细胞，以检测受损的细胞，这些细胞是活的，但使用基于平板的方法是不可培养的，并评估牛奶热处理对这些群体的影响。使用死细胞悬浮液以及热处理和非热处理的细胞悬浮液。在60 ° C或65 °C处理后，受损细胞的数量增加，这是由于具有受损膜的细胞从热处理的悬浮液中转移的结果。这些温度并不能有效杀死所有细菌，但能有效破坏它们的细胞膜。因此，温和的热处理不足以保证婴儿配方奶粉的安全性。流式细胞术检测C.因此，它可以作为一种筛选测试，以降低这种用于新生儿营养的食品中存在致病性活细胞但不可培养细胞的风险。
Cronobacter sakazakii is an opportunistic pathogen that is associated with outbreaks of neonatal necrotizing enterocolitis, septicaemia, and meningitis. Reconstituted powdered infant formulae is the most common vehicle of infection. The aim of the present study is to gain insight into the physiological states of C. sakazakii cells using flow cytometry to detect the compromised cells, which are viable but non-culturable using plate-based methods, and to evaluate the impact of milk heat treatments on those populations. Dead-cell suspensions as well as heat-treated and non-heat-treated cell suspensions were used. After 60 or 65 °C treatments, the number of compromised cells increased as a result of cells with compromised membranes shifting from the heat-treated suspension. These temperatures were not effective at killing all bacteria but were effective at compromising their membranes. Thus, mild heat treatments are not enough to guarantee the safety of powered infant formulae. Flow cytometry was capable of detecting C. sakazakii’s compromised cells that cannot be detected with classical plate count methods; thus, it could be used as a screening test to decrease the risk derived from the presence of pathogenic viable but non-culturable cells in this food that is intended for newborns’ nutrition.