Biological and protein-binding studies of newly synthesized polymer-cobalt(III) complexes.
Biological and protein-binding studies of newly synthesized polymer-cobalt(III) complexes.
复制标题
DOI：
10.1002/bio.2992
复制
发表时间：
2016-03
期刊：
影响因子：
--
通讯作者：
G. Vignesh;I. Pradeep;S. Arunachalam;S. Vignesh;R. A. James;R. Arun;Kumpati Premkumar
中科院分区：
文献类型：
--
作者：
G. Vignesh;I. Pradeep;S. Arunachalam;S. Vignesh;R. A. James;R. Arun;Kumpati Premkumar
合成并表征了聚合物-钴(III)配合物[Co(bpy)(dien)BPEI]Cl3·4H2O（bpy = 2,2'-联吡啶，dien = 二乙烯三胺，BPEI = 支化聚乙烯亚胺）。使用各种物理化学技术在生理条件下研究了这些复合物与人血清白蛋白（HSA）和牛血清白蛋白（BSA）的相互作用。结果表明，聚合物-钴(III)复合物对血清白蛋白的荧光猝灭是通过静态猝灭发生的。这些复合物的结合极大地改变了蛋白质的分子构象。 x = 0.365 的聚合物-钴(III) 复合物显示出针对多种人类病原体的抗菌活性。该复合物还通过诱导细胞凋亡诱导针对 MCF-7 的细胞毒性。然而，还需要进一步的研究来破译聚合物-钴（III）复合物的分子作用模式及其在抗癌治疗中的可能用途。
The polymer-cobalt(III) complexes, [Co(bpy)(dien)BPEI]Cl3 · 4H2O (bpy = 2,2'-bipyridine, dien = diethylentriamine, BPEI = branched polyethyleneimine) were synthesized and characterized. The interaction of these complexes with human serum albumin (HSA) and bovine serum albumin (BSA) was investigated under physiological conditions using various physico-chemical techniques. The results reveal that the fluorescence quenching of serum albumins by polymer-cobalt(III) complexes took place through static quenching. The binding of these complexes changed the molecular conformation of the protein considerably. The polymer-cobalt(III) complex with x = 0.365 shows antimicrobial activity against several human pathogens. This complex also induces cytotoxicity against MCF-7 through apoptotic induction. However, further studies are needed to decipher the molecular mode of action of polymer-cobalt(III) complex and for its possible utilization in anticancer therapy.