Co-activation of synovial fibroblasts by laminin-111 and transforming growth factor-β induces expression of matrix metalloproteinases 3 and 10 independently of nuclear factor-κB
Co-activation of synovial fibroblasts by laminin-111 and transforming growth factor-β induces expression of matrix metalloproteinases 3 and 10 independently of nuclear factor-κB
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层粘连蛋白 111 和转化生长因子 β 共同激活滑膜成纤维细胞诱导基质金属蛋白酶 3 和 10 的表达，独立于核因子 βB
DOI：
10.1136/ard.2007.073809
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发表时间：
2008
影响因子：
27.4
通讯作者：
W.K. Aicher
中科院分区：
文献类型：
--
作者：
K. Warstat;T. Pap;G. Klein;S. Gay;W.K. Aicher
我们之前的研究表明，在转化生长因子-β存在的情况下，滑膜成纤维细胞附着在层粘连蛋白(LM)-111上，诱导基质金属蛋白酶(MMP)-3的显著表达。在这里，我们继续研究其他MMPs及其特异性基质蛋白酶组织抑制剂（TIMPs）的调节。通过定量逆转录-聚合酶链反应研究编码TIMPs和MMPs的稳态mRNA水平的变化。多路免疫阵列监测MMPs的产生。免疫印迹法研究信号转导通路。在转化生长因子-β存在的情况下，将滑膜成纤维细胞附着在dm -111上，诱导MMP-3 mRNA（12.35倍，p<0.001）和蛋白质（平均62 ng/ml， 6倍，p<0.008）的显著增加，以及MMP-10 mRNA（11.68倍，p<0.05）和蛋白质（54 ng/ml， 20倍，p小于0.02）的表达显著增加。所有其他TIMPs和MMPs都没有表现出lm -111促进的转化生长因子-β反应。核因子-κB未见磷酸化。我们得出结论，LM-111和转化生长因子-β共同刺激滑膜成纤维细胞足以诱导MMP-3和MMP-10在滑膜成纤维细胞中的显著表达，并且这种诱导不依赖于核因子-κB磷酸化。
We showed previously that the attachment of synovial fibroblasts to laminin (LM)-111 in the presence of transforming growth factor-β induces significant expression of the matrix metalloproteinase (MMP)-3. Here we go on to investigate the regulation of additional MMPs and their specific tissue inhibitors of matrix proteases (TIMPs). Changes in steady-state mRNA levels encoding TIMPs and MMPs were investigated by quantitative reverse transcription–polymerase chain reaction. Production of MMPs was monitored by a multiplexed immunoarray. Signal transduction pathways were studied by immunoblotting. Attachment of synovial fibroblasts to LM-111 in the presence of transforming growth factor-β induced significant increases in MMP-3 mRNA (12.35-fold, p<0.001) and protein (mean 62 ng/ml, sixfold, p<0.008) and in expression of MMP-10 mRNA (11.68-fold, p<0.05) and protein (54 ng/ml, 20-fold, p⩾0.02). All other TIMPs and MMPs investigated failed to show this LM-111-facilitated transforming growth factor-β response. No phosphorylation of nuclear factor-κB was observed. We conclude that co-stimulation of synovial fibroblasts by LM-111 together with transforming growth factor-β suffices to induce significant expression of MMP-3 and MMP-10 by synovial fibroblasts and that this induction is independent of nuclear factor-κB phosphorylation.
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影响因子：
27.4
作者：
Drynda, S;Kühne, C;Kekow, J
通讯作者：
Kekow, J
滑膜成纤维细胞生长因子 ß 诱导的基质表达中的金属蛋白酶 3 附着于层粘连蛋白 111 促进转化
DOI：
--
发表时间：
2008
期刊：
影响因子：
--
作者：
K. Aicher;M. Hoberg;M. Rudert;T. Pap;G. Klein
通讯作者：
G. Klein
一种测量基质金属蛋白酶在关节炎患者滑液中总体净蛋白水解活性中的贡献的新方法
影响因子：
4.9
作者：
N. Simard;G. Boire;A. D. de Brum;Y. St
通讯作者：
Y. St
影响因子：
--
作者：
Mengshol, JA;Vincenti, MP;Brinckerhoff, CE
通讯作者：
Brinckerhoff, CE