Role of dendritic cells in the priming of CD4+ T lymphocytes to peptide antigen in vivo.
Role of dendritic cells in the priming of CD4+ T lymphocytes to peptide antigen in vivo.
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DOI：
10.4049/jimmunol.151.12.6742
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发表时间：
1993-12
影响因子：
4.4
通讯作者：
D. Levin;Stephanie L. Constant;T. Pasqualini;R. A. Flavell;K. Bottomly
中科院分区：
文献类型：
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作者：
D. Levin;Stephanie L. Constant;T. Pasqualini;R. A. Flavell;K. Bottomly
检测了树突状细胞（DC）在体内CD 4 + T淋巴细胞初始引发中的贡献。为了评估不同APC致敏CD 4 + T细胞的能力，利用一系列在APC上差异表达I-E的MHC II类I-E转基因小鼠。在体内用I-E限制性肽致敏主要在DC、巨噬细胞和B细胞上表达I-E的转基因小鼠，并通过用相同的Ag体外再刺激CD 4 + T细胞来确定致敏的程度。结果表明，DC是启动CD 4 + T细胞所必需的，并且启动的程度与I-E+ DC的频率相关。此外，单独的DC可以用作肽Ag的APC，因为通过肽脉冲的I-E+ DC的转移可以在I-E阴性小鼠中恢复引发。与B细胞和巨噬细胞相比，DC对初始CD 4 + T细胞的效力通过体外研究得到证实。
The contribution of dendritic cells (DC) in the initial priming of CD4+ T lymphocytes in vivo was examined. To assess the capacity of different APC to prime CD4+ T cells, a series of MHC class II I-E transgenic mice that differentially express I-E on APC were utilized. Transgenic mice that express I-E primarily on DC, on macrophages, and on B cells were primed with an I-E restricted peptide in vivo, and the extent of priming was determined by restimulation of CD4+ T cells in vitro with the same Ag. The results indicate that DC are required for priming of CD4+ T cells, and that the extent of priming correlates with the frequency of I-E+ DC. Moreover, DC alone can serve as APC for the peptide Ag, in that priming can be restored in an I-E negative mouse by the transfer of peptide-pulsed I-E+ DC. The potency of DC, compared with B cells and macrophages, to prime naive CD4+ T cells was confirmed with in vitro studies.