MHC-DRB1/DQB1 Gene Polymorphism and Its Association with Resistance/Susceptibility to Cystic Echinococcosis in Chinese Merino Sheep.
MHC-DRB1/DQB1 Gene Polymorphism and Its Association with Resistance/Susceptibility to Cystic Echinococcosis in Chinese Merino Sheep.
复制标题
中国美利奴羊MHC-DRB1/DQB1基因多态性及其与包虫病抗性/易感性的关系。
DOI：
10.1155/2014/272601
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发表时间：
2014
影响因子：
2.2
通讯作者：
Du Y
中科院分区：
文献类型：
--
作者：
Shen H;Han G;Jia B;Jiang S;Du Y
本研究旨在分析中国美利奴羊MHC-DRB 1/DQB 1基因多态性与抗包虫病的关系，筛选抗包虫病的分子遗传标记。应用聚合酶链反应（PCR）技术，从健康绵羊和包虫病绵羊的DNA中扩增出MHCII-DRB 1/DQB 1外显子2。用限制性片段长度多态性（RFLP）技术对PCR产物进行鉴定。五种限制酶（Mval、Hae III、SacI、SacII和Hin 1 I）用于切割DRB 1，而七种限制性内切酶（Mval、Hae III、SacI、SacII和Hin 1 I）用于切割DRB 1。用MroxI、ScaI、SacII、NciI、TaqI、Mval和HaeIII酶切DQB 1。结果表明，Mvalbb酶切模式频率与DQB 1酶切模式频率呈负相关。DRB 1外显子2的SacIab阳性率显著高于对照组（P < 0.01（P < 0.05），TaqIaa、HaeIIInn DQB 1外显子2的基因型频率在健康组显著高于对照组（P < 0.01），提示这些基因型与包虫病的耐药或易感性有很强的相关性。卡方检验表明，单倍型DRB 1-SacIab/DRB 1-Mvalbb/DQB 1-TaqIaa/DQB 1-HaeIIInn（P < 0.01）的个体对C.E相对抗性，而单倍型DRB 1-Mvalbc/DQB 1-Mvalyy/DQB 1-TaqIab/DQB 1-HaeIIImn（P < 0.01）和DRB 1-Mvalbb/DQB 1-Mvalcc/DQB 1-TaqIab/DQB 1-HaeIIImn（P < 0.01）的个体对C. E较敏感。此外，为了证实这些结果，用人工感染E.g.的中国美利奴羊进行了田间试验，结果与第一次研究的结果一致。因此，中国美利奴羊MHC-DRB 1/DQB 1外显子2在抗大肠杆菌中起重要作用。另外，在中国美利奴羊中筛选出抗包虫病的分子遗传标记（DRB 1-SacIab/DRB 1-Mvalbb/DQB 1-TaqIaa/DQB 1-HaeIIInn）。
The aim of this study was to analyze the relationship between polymorphism of the MHC-DRB1/DQB1 gene and its resistance to Cystic Echinococcosis (C.E), as well as to screen out the molecular genetic marker of antiechinococcosis in Chinese Merino sheep. The MHCII-DRB1/DQB1 exon 2 was amplified by polymerase chain reaction (PCR) from DNA samples of healthy and hydatidosis sheep. PCR products were characterized by restriction fragment length polymorphism (RFLP) technique. Five restriction enzymes (Mval, HaeIII, SacI, SacII, and Hin1I) were employed to cut DRB1, while seven restriction enzymes (MroxI, ScaI, SacII, NciI, TaqI, Mval, and HaeIII) were employed to cut DQB1.Results showed that frequencies of patterns Mvalbb (P < 0.01), SacIab in DRB1 exon 2 (P < 0.05), and TaqIaa, HaeIIInn (P < 0.01) in DQB1 exon 2 were significantly higher in the healthy group compared with the C.E individuals, which implied that there was a strong association between these genotypes and hydatidosis resistance or susceptibility. Chi-square test showed that individuals with the genic haplotype DRB1-SacIab/DRB1-Mvalbb/DQB1-TaqIaa/DQB1-HaeIIInn (P < 0.01) were relatively resistant to C.E, while individuals with the genic haplotypes DRB1-Mvalbc/DQB1-Mvalyy/DQB1-TaqIab/DQB1-HaeIIImn (P < 0.01) and DRB1-Mvalbb/DQB1-Mvalcc/DQB1-TaqIab/DQB1-HaeIIImn (P < 0.01) were more susceptible to C.E. In addition, to confirm these results, a fielding experiment was performed with Chinese Merino sheep which were artificially infected with E.g. The result was in accordance with the results of the first study. In conclusion, MHC-DRB1/DQB1 exon 2 plays an important role as resistant to C.E in Chinese Merino sheep. In addition, the molecular genetic marker of antiechinococcosis (DRB1-SacIab/DRB1-Mvalbb/DQB1-TaqIaa/DQB1-HaeIIInn) was screened out in Chinese Merino sheep.