Microbubbles as biocompatible porogens for hydrogel scaffolds.
Microbubbles as biocompatible porogens for hydrogel scaffolds.
复制标题
微泡作为水凝胶支架的生物相容性致孔剂。
DOI：
10.1016/j.actbio.2012.07.007
复制
发表时间：
2012
影响因子：
9.7
通讯作者：
Hung,ClarkT
中科院分区：
文献类型：
--
作者：
Lima,EricG;Durney,KristaM;Sirsi,ShashankR;Nover,AdamB;Ateshian,GerardA;Borden,MarkA;Hung,ClarkT
在这项研究中，我们探讨了应用脂质壳，充气微泡作为一种方法，创造按需微孔水凝胶软骨组织工程。该技术允许细胞和微孔在支架内均匀分布，以浓度依赖性方式增加大溶质（70kDa葡聚糖）的吸收系数。微泡的气相稳定性取决于几个因素，包括微泡悬浮液的初始尺寸分布，以及培养过程中的温度和压力。压力循环的应用提供了气相的受控释放，以产生具有残余脂质的流体填充的微孔。所得的微孔琼脂糖支架具有生物相容性，在42天的培养期内，工程化软骨特性增加了两倍（气泡组的EY=492±42kPa，无气泡对照组的EY = 249±49kPa）。我们的研究结果表明，微泡提供了一种简单而强大的方法，通过温和的加压调节细胞接种水凝胶中的传质，该方法可能会在未来扩展到包括聚焦超声，以改善时空控制。
In this study, we explored the application of lipid-shelled, gas-filled microbubbles as a method for creating on-demand microporous hydrogels for cartilage tissue engineering. The technique allowed for homogenous distribution of cells and micropores within the scaffold, increasing the absorption coefficient of large solutes (70kDa dextran) over controls in a concentration-dependent manner. The stability of the gas phase of the microbubbles depended on several factors, including the initial size distribution of the microbubble suspension, as well as the temperature and pressure during culture. Application of pressure cycles provided controlled release of the gas phase to generate fluid-filled micropores with remnant lipid. The resulting microporous agarose scaffolds were biocompatible, leading to a twofold increase in engineered cartilage properties (EY=492±42kPa for the bubble group vs. 249±49kPa for the bubble-free control group) over a 42-day culture period. Our results suggest that microbubbles offer a simple and robust method of modulating mass transfer in cell-seeded hydrogels through mild pressurization, and the methodology may be expanded in the future to include focused ultrasound for improved spatio-temporal control.