Distinguishing Between Endodermal and Pluripotent Stem Cell Lines During Somatic Cell Reprogramming.
Distinguishing Between Endodermal and Pluripotent Stem Cell Lines During Somatic Cell Reprogramming.
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DOI：
10.1007/978-1-0716-1979-7_4
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发表时间：
2022-01-01
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影响因子：
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通讯作者：
Ralston, A
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文献类型：
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作者：
Moauro, A;Ralston, A
使用 Oct4、Sox2、Klf4 和 c-Myc (OSKM) 的小鼠体细胞重编程可诱导两种干细胞类型的形成：诱导多能干 (iPS) 细胞和诱导胚胎外内胚层干 (iXEN) 细胞。由于两种干细胞类型在重编程过程中通常会同时出现，因此区分这两种细胞类型以确保选择和分析所需的细胞群至关重要。本章从头到尾详细介绍了如何使用逆转录病毒对小鼠胚胎成纤维细胞 (MEF) 进行重编程，以及如何在集落和单细胞水平上区分 iXEN 和 iPS 细胞。
Mouse somatic cell reprogramming using Oct4, Sox2, Klf4 and c-Myc (OSKM) induces formation of two stem cell types: induced pluripotent stem (iPS) cells and induced extraembryonic endoderm stem (iXEN) cells. Since both stem cells types routinely arise alongside one another during reprogramming, it is critical to distinguish between both cell types to ensure that the desired cell population is selected and analyzed. This chapter details, from start to finish, how to reprogram mouse embryonic fibroblasts (MEFs) using retrovirus and how to distinguish between iXEN and iPS cells at the colony and single-cell levels.