Establishment of an Enzyme-Linked Immunosorbent Assay and Application on Determination of Ginsenoside Re in Human Saliva
Establishment of an Enzyme-Linked Immunosorbent Assay and Application on Determination of Ginsenoside Re in Human Saliva
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酶联免疫吸附法的建立及其在测定人唾液中人参皂苷Re的应用
DOI：
10.1055/s-0034-1382959
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发表时间：
2014-08-01
期刊：
影响因子：
2.7
通讯作者：
Wang, Qingguo
中科院分区：
文献类型：
--
作者：
Qu, Huihua;Sai, Jiayang;Wang, Qingguo
本文介绍了人参质量控制的免疫化学方法和人参皂苷Re的药理研究。人参皂苷Re是人参中的主要生物活性成分。将人参皂苷Re-牛血清白蛋白结合物免疫的脾细胞与次黄嘌呤-氨基蝶呤-胸腺嘧啶敏感的小鼠骨髓瘤SP2/0细胞融合，获得分泌人参皂苷Re单抗的杂交瘤细胞株。该方法在人参皂苷Re的有效测量范围7.8~500ng.mL(-1)范围内，成功地测定了中药处方中人参皂苷Re的含量。结果表明，我们建立了一种新的、可靠的测定系统，可以更有效地测定中药中人参皂苷Re。此外，我们还测定了6名健康成人口服人参胶囊后唾液中人参皂苷Re的浓度，以研究人参皂苷Re在人唾液中的药代动力学。
This work describes an immunochemical approach for the quality control of Panax ginseng and a pharmacological study of ginsenoside Re, a major bioactive constituent in P. ginseng, using an enzyme-linked immunosorbent assay. A hybridoma secreting monoclonal antibody against ginsenoside Re was produced by fusing splenocytes immunized with a ginsenoside Re-bovine serum albumin conjugate with the hypoxanthine-aminopterin-thymidine-sensitive mouse myeloma SP2/0 cell line. The method, at an effective measuring range of 7.8-500 ng.mL(-1) of ginsenoside Re, successfully detected ginsenoside Re in Chinese traditional herb prescriptions. The results demonstrate that we generated a novel and reliable assay system for measuring ginsenoside Re in Chinese medicines more efficiently. Futhermore, we determined the ginsenoside Re concentrations in the saliva of six healthy adults after the oral administration of a ginseng capsule to study the pharmacokinetics of ginsenoside Re in human saliva.