Effects of vasopressin on phosphoinositides and prostaglandin production in cultured mesangial cells.
Effects of vasopressin on phosphoinositides and prostaglandin production in cultured mesangial cells.
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加压素对培养的系膜细胞中磷酸肌醇和前列腺素产生的影响。
DOI：
10.1152/ajprenal.1985.249.1.f139
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Venkatachalam,MA
中科院分区：
文献类型：
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作者：
Troyer,DA;Kreisberg,JI;Schwertz,DW;Venkatachalam,MA
我们研究了加压素对系膜细胞磷脂代谢的影响，以及这些变化与前列腺素产生的时间关系。这些变化包括：1）磷脂酰肌醇（PI）的分解增加，2）二磷酸肌醇（PI-P）和三磷酸肌醇（PI-P2）的分解增加，3）甘油二酯和磷脂酸的质量增加，4）合成增加，首先是PI-P和PI-P2，然后是磷脂酸，最后是PI，和5）水溶性肌醇磷酸增加。加压素治疗导致磷脂酸质量显著增加（90%），PI质量下降较小（13%）。血管加压素治疗45 s后观察到甘油二酯的变化。磷脂酰肌醇代谢的改变，早在45秒，并持续长达5小时后激素暴露。相比之下，前列腺素的生产下降30分钟后。这些观察加压素刺激的代谢在培养的系膜细胞可能提供一个基础，了解激素暴露后的功能变化。
We studied the effects of vasopressin on phospholipid metabolism in mesangial cells and the temporal relationship of these changes to prostaglandin production. The changes included: 1) increased breakdown of phosphatidylinositol (PI), 2) increased breakdown of diphosphoinositide (PI-P) and triphosphoinositide (PI-P2), 3) an increase in the mass of diglyceride and phosphatidic acid, 4) increased synthesis, first of PI-P and PI-P2, then phosphatidic acid, and, finally, PI, and 5) increased water-soluble inositol phosphates. Vasopressin treatment resulted in a significant increase (90%) in the mass of phosphatidic acid and a smaller (13%) decline in the mass of PI. Changes in diglyceride were seen following 45 s of treatment with vasopressin. Alterations of phosphoinositide metabolism were seen as early as 45 s and continued for up to 5 h following hormone exposure. By contrast, prostaglandin production declined after 30 min. These observations on vasopressin-stimulated metabolism in cultured mesangial cells may provide a basis for an understanding of the functional changes that follow hormone exposure.