A New Sensitive, Whole-Cell Hybridization Technique for Detection of Bacteria Involving a Biotinylated Oligonucleotide Probe Targeting rRNA and Tyramide Signal Amplification
A New Sensitive, Whole-Cell Hybridization Technique for Detection of Bacteria Involving a Biotinylated Oligonucleotide Probe Targeting rRNA and Tyramide Signal Amplification
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一种用于检测细菌的新型灵敏全细胞杂交技术，涉及靶向 rRNA 和酪酰胺信号放大的生物素化寡核苷酸探针
DOI：
10.1128/aem.63.8.3274-3278.1997
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发表时间：
1997
影响因子：
4.4
通讯作者：
L. Bernard
中科院分区：
文献类型：
--
作者：
P. Lebaron;P. Catala;C. Fajon;F. Joux;J. Baudart;L. Bernard
利用生物素化寡核苷酸探针和链亲和素-辣根过氧化物酶组成的酪酰胺信号扩增系统，提高荧光原位杂交技术的灵敏度。当应用于固定在玻璃载玻片上的革兰氏阴性和阳性细菌时，观察到荧光信号相对于用荧光单标记探针杂交的细胞扩增7至12倍。在饥饿条件下和来自海洋环境的自然样品中可以检测到很大比例（62%至78%）的细菌。这种放大程序允许对海洋少营养生态系统和水质控制进行新的调查。
A tyramide signal amplification system with biotinylated oligonucleotide probes and streptavidin-horseradish peroxidase was used to increase the sensitivity of fluorescent in situ hybridization techniques. When applied to both gram-negative and -positive bacteria immobilized on glass slides, a 7- to 12-fold amplification of the fluorescence signal was observed relative to that of cells hybridized with fluorescently monolabeled probes. A large proportion (62 to 78%) of bacteria could be detected under starvation conditions and in natural samples from the marine environment. This amplification procedure allows new investigations in marine oligotrophic ecosystems and water quality control.