The human gene AHNAK encodes a large phosphoprotein located primarily in the nucleus.
The human gene AHNAK encodes a large phosphoprotein located primarily in the nucleus.
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DOI：
10.1083/jcb.120.3.625
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发表时间：
1993-02
期刊：
影响因子：
--
通讯作者：
Bishop JM
中科院分区：
文献类型：
--
作者：
Shtivelman E;Bishop JM
AHNAK是一种新发现的人类基因，以其特殊的大小而闻名（c.a. 700 kD）及其产物的结构，以及抑制其在人神经母细胞瘤细胞中的表达。在这里，我们报告的AHNAK编码的蛋白质的鉴定和部分表征。该蛋白主要（但不完全）位于细胞核中，并在丝氨酸和苏氨酸上磷酸化。当细胞退出分裂周期时，蛋白质的丰度明显增加，响应于血清（成纤维细胞）或分化（神经母细胞瘤细胞）的撤回。相比之下，磷酸化的量似乎在这些环境中减少。AHNAK蛋白的大量存在和纤维状结构表明其在细胞结构中起作用，但其功能尚不清楚。
AHNAK is a newly identified human gene notable for the exceptional size (c.a. 700 kD) and structure of its product, and for the repression of its expression in human neuroblastoma cells. Here we report the identification and partial characterization of the protein encoded by AHNAK. The protein is located principally (but not exclusively) in the nucleus and is phosphorylated on both serine and threonine. The abundance of the protein increases appreciably when cells withdraw from the division cycle, in response to either withdrawal of serum (fibroblasts) or differentiation (neuroblastoma cells). By contrast, the amount of phosphorylation appears to diminish in those settings. The considerable abundance and conjectured fibrous structure of AHNAK protein suggest a role in cytoarchitecture, but no function can yet be discerned.