Membranes of the adrenal medulla. Behaviour of insoluble proteins of chromaffin granules on gel electrophoresis.
Membranes of the adrenal medulla. Behaviour of insoluble proteins of chromaffin granules on gel electrophoresis.
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肾上腺髓质膜。
DOI：
10.1042/bj1180303
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发表时间：
1970
期刊：
影响因子：
--
通讯作者：
A. D. Smith
中科院分区：
文献类型：
--
作者：
H. Winkler;H. Hörtnagl;A. D. Smith
牛肾上腺嗜铬颗粒的洗涤膜含有颗粒的大部分胆固醇和磷脂以及总蛋白的22%。蛋白质/脂质比为约0.45（w/w）。多巴胺（3，4-二羟基苯乙胺）β-羟化酶、Mg（2+）激活的核苷三磷酸酶和细胞色素B-559活性存在于膜中。ATP是核苷三磷酸酶的最适底物，其最适pH为6.4，K（m）为7 × 10 ~（-4）m，V（max）为1.0 × 10 ~（-4）m。1.8 μ mol/h/mg蛋白质。用各种洗涤剂处理的膜引起蛋白质与脂质相比的优先溶解。将膜溶解在十二烷基硫酸钠或苯酚-乙酸-尿素中，分别在碱性和酸性pH下进行聚丙烯酰胺凝胶电泳。嗜铬颗粒膜蛋白质的电泳模式不同于嗜铬粒蛋白，以及线粒体和微粒体膜蛋白。
Washed membranes of bovine adrenal chromaffin granules contained most of the cholesterol and phospholipids of the particle and 22% of the total protein. The protein/lipid ratio was about 0.45 (w/w). Dopamine(3,4-dihydroxyphenethylamine)beta-hydroxylase, Mg(2+)-activated nucleoside triphosphatase and cytochrome b-559 activities were present in the membrane. ATP was the best substrate for the nucleoside triphosphatase, whose pH optimum was 6.4, K(m) 7x10(-4)m and V(max.) 1.8mumol/h per mg of protein. Treatment of the membranes with various detergents caused a preferential solubilization of protein compared with lipids. Membranes dissolved in sodium dodecyl sulphate or phenol-acetic acid-urea were subjected to polyacrylamide-gel electrophoresis at alkaline and acid pH respectively. The electrophoretic patterns given by the proteins of the chromaffin granule membrane were distinct from those given by the chromogranins, and from those given by mitochondrial and microsomal membrane proteins.