Differential activity and degradation of plant volatile elicitors in regurgitant of tobacco hornworm (Manduca sexta) larvae
Differential activity and degradation of plant volatile elicitors in regurgitant of tobacco hornworm (Manduca sexta) larvae
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DOI：
10.1023/a:1024209302628
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发表时间：
2003-06-01
影响因子：
2.3
通讯作者：
Tumlinson, JH
中科院分区：
文献类型：
--
作者：
Alborn, HT;Brennan, MM;Tumlinson, JH
植物对昆虫食草的反应是释放挥发性化合物，吸引食草动物的天敌。植物中许多挥发物的生物合成是由食草动物产生的化合物诱导的。研究了烟草角虫（THW）反流剂各成分对玉米幼苗挥发物的诱导作用。分离鉴定出挥发性物质释放强度最大的两种成分为n -亚麻烯酰-l -谷氨酰胺（18:3-GLN）和n -亚麻烯酰-l -谷氨酸（18:3-GLU）。在其他几种鳞翅目幼虫的反刍物中也发现了大约10倍活性的18:3-GLN，当THW反刍物放在室温下时，其降解速度很快，而18:3-GLU的降解速度要慢得多。不同饲粮来源（包括寄主植物和非寄主植物）对反刍物中脂肪酸-氨基酸偶联物的氨基酸组成没有影响。
Plants respond to insect herbivory by emitting volatile compounds that attract natural enemies of the herbivores. Biosynthesis of many of these volatiles in plants is induced by herbivore-produced compounds. Components of tobacco hornworm (THW) regurgitant were investigated for their efficacy as elicitors of corn seedling volatiles. Two components that elicited the strongest release of volatiles were isolated and identified as N-linolenoyl-L-glutamine (18:3-GLN) and N-linolenoyl-L-glutamic acid (18:3-GLU). The approximately 10 times more active 18:3-GLN, which also is found in the regurgitant of several other Lepidopteran larvae, was rapidly degraded when THW regurgitant was left at room temperature, while 18:3-GLU degraded at a much slower rate. Different dietary sources of THW and tobacco bud worm larvae, including both host and nonhost plants, did not affect the amino acid composition of the fatty acid-amino acid conjugates in the regurgitant.