Neuronal localization of specific angiotensin II binding sites in the rat inferior olivary nucleus.
Neuronal localization of specific angiotensin II binding sites in the rat inferior olivary nucleus.
复制标题
大鼠下橄榄核中特定血管紧张素 II 结合位点的神经元定位。
DOI：
10.1111/j.1471-4159.1988.tb02985.x
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发表时间：
1988
影响因子：
4.7
通讯作者：
Speth,RC
中科院分区：
文献类型：
--
作者：
Walters,DE;Speth,RC
成年雄性Sprague-道利大鼠接受3-乙酰吡啶（一种对下橄榄核具有选择性的神经毒素）处理。给药后，大鼠表现出运动行为缺陷，表明下橄榄核遭到破坏。3周后处死大鼠，测定125 I-肌氨酸1、异亮氨酸8-血管紧张素II与脑匀浆和脑干载玻片切片的结合。用3-乙酰吡啶处理可显著降低脑干后腹侧匀浆（含下橄榄核）中特异性125 I-肌氨酸、异亮氨酸8-血管紧张素II结合，降低幅度约为50%。背侧脑干和下丘脑-丘脑-中脑匀浆的特异性结合无显著变化。用3-乙酰吡啶处理未显著改变在下丘脑-丘脑-中脑中测定的放射性配体结合亲和力。脑干切片中125 I-肌氨酸1、异亮氨酸8血管紧张素II结合的放射自显影分析表明，下橄榄核中的特异性血管紧张素II结合位点实际上被3-乙酰吡啶处理消除。此外，在孤束核/迷走神经背侧运动核复合体中，特异性125 I-肌氨酸1、异亮氨酸8血管紧张素II结合发生了相对较小但显著的降低。这些结果表明，特定的血管紧张素II结合位点的下橄榄核只发生在神经元胞体和/或树突。
Adult male Sprague‐Dawley rats were treated with 3‐acetylpyridine, a neurotoxin selective for the inferior olivary nucleus. Following treatment, the rats exhibited deficits in locomotor behavior indicative of destruction in the inferior olivary nucleus. The rats were sacrificed 3 weeks later, and the binding of125I‐sarcosine1, isoleucine8angiotensin II to brain homogenates and slide‐mounted sections of brainstem was determined. Treatment with 3‐acetylpyridine significantly decreased specific125I‐sarco‐sine', isoleucine8angiotensin II binding in homogenates of posteroventral brainstem (containing the inferior olivary nucleus) by approximately 50%. Homogenates of dorsal brainstem and hypothalamus‐thalamus‐midbrain showed no significant changes in specific binding. Treatment with 3‐acetylpyridine did not significantly alter the radioligand binding affinity which was determined in the hypothalamus‐thalamus‐midbrain. Autoradiographic analysis of125I‐sarcosine1, isoleucine8angiotensin II binding in the brainstem sections indicated that specific angiotensin II binding sites in the inferior olivary nucleus were virtually eliminated by the 3‐acetylpyridine treatment. In addition, a comparatively small, but significant, decrease in specific125I‐sarcosine1, isoleucine8angiotensin II binding occurred in the solitary tract nucleus/dorsal vagal motor nucleus complex. These results indicate that specific angiotensin II binding sites in the inferior olivary nucleus occur exclusively on neuronal perikarya and/or dendrites.